An in vivo analysis of Cr6+ induced biochemical, genotoxicological and transcriptional profiling of genes related to oxidative stress, DNA damage and apoptosis in liver of fish, Channa punctatus (Bloch, 1793)

Aquatic Toxicology
Yashika AwasthiSunil P Trivedi

Abstract

Present study was designed to assess the hexavalent chromium (Cr6+) mediated oxidative stress that induces DNA damage and apoptosis in adult fish, Channa punctatus (35 ± 3.0 g; 14.5 ± 1.0 cm; Actinopterygii). Fishes were maintained in three groups for 15, 30 and 45 d of exposure periods. They were treated with 5% (Group T1) and 10% (Group T2) of 96 h-LC50 of chromium trioxide (Cr6+). Controls were run for the similar duration. A significant (p < 0.05) increment in the activities of antioxidant enzymes, SOD and CAT in liver tissues of the exposed fish evinces the persistence of oxidative stress. A significant (p < 0.05) increase in induction of micronuclei (MN) coupled with transcriptional responses of target genes related to antioxidant enzymes, DNA damage and apoptosis (sod, cat, gsr, nox-1, p53, bax, bcl-2, apaf-1 and casp3a) establishes the impact of oxidative stress due to in vivo, Cr6+ accumulation in liver as compared to control (0 mg/L), in a dose and exposure-dependent manner. Initially, the increased level of reactive oxygen species (ROS) in liver coincided with that of enhanced mRNA expression of antioxidant enzymes, sod, cat, gsr and nox-1 but, later, the overproduction of ROS, after 45 d of exposure of Cr6+, resulte...Continue Reading

Citations

Jan 12, 2019·Bulletin of Environmental Contamination and Toxicology·Emanuelle Carneiro QueirozAdriana Regina Chippari-Gomes
May 3, 2019·Biological Trace Element Research·Manoj KumarSunil P Trivedi
Feb 17, 2019·Bulletin of Environmental Contamination and Toxicology·Michel GallãoAdriana R Chippari-Gomes
Mar 25, 2021·Environmental Toxicology and Pharmacology·Yiran ZhuJianzhu Liu
Apr 20, 2021·Chemical Communications : Chem Comm·Nitika PradhanPrasanta K Maiti
Aug 23, 2019·Chemical Research in Toxicology·Bei ChenYu-Qi Feng
Oct 30, 2021·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Qixia ChanYuzhe Han

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