PMID: 7581520Nov 1, 1995Paper

An in vivo analysis of transcriptional elements in the mouse alpha-myosin heavy chain gene promoter

Transgenic Research
H RindtJ Robbins

Abstract

During development in the murine ventricle, there is a switch in myosin heavy chain gene (MyHC) transcription. The beta-MyHC is expressed in the ventricles during foetal development, but is shut down at or around birth, at which time alpha-MyHC transcription is activated. This antithetical switch is thought to be mediated by circulating levels of thyroid hormone (TH) and both low and high affinity thyroid response elements (TREs) have been identified in the proximal promoter region of the murine alpha-MyHC. Myosin gene expression in the atria is relatively unaffected by the TH status. Previously, we used site-directed mutagenesis of the promoter in a transgenic analysis to define those elements responsible for high levels of transcription in vivo. These analyses focused on the role(s) of two cis elements, TRE1 and TRE2 that are located at -129 to -149 and -102 to -120, respectively, on the alpha-MyHC promoter. Although the elements' ablation had differential effects on transgene expression, neither single mutation abolished transgene expression completely. Here, we show that mutating both elements results in a complete inactivation of the transgene in both ventricles and atria under euthyroid conditions. However, expression sti...Continue Reading

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Citations

Aug 1, 1997·Trends in Cardiovascular Medicine·J Robbins
Jun 9, 2000·Annual Review of Physiology·J Robbins
Feb 14, 1998·The Journal of Clinical Investigation·M A SussmanT R Kimball
Feb 7, 2004·Proceedings of the National Academy of Sciences of the United States of America·Ralph V ShohetMasashi Yanagisawa
Apr 7, 2010·The Journal of Cell Biology·David S GokhinVelia M Fowler
Apr 22, 2014·Journal of Molecular and Cellular Cardiology·Wenjuan WangTaixing Cui
May 12, 2004·Journal of Molecular and Cellular Cardiology·Jeffrey Robbins
Oct 23, 1997·Developmental Dynamics : an Official Publication of the American Association of Anatomists·L LefaucheurA Kelly
Mar 20, 2004·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Dirk WernickeLudwig Thierfelder
Jul 31, 2003·Developmental Dynamics : an Official Publication of the American Association of Anatomists·William E TidymanCharles P Ordahl
Mar 1, 1996·Circulation Research·J PalermoJ Robbins
Oct 1, 1996·Circulation Research·Z YuG I Fishman
Mar 19, 2016·Circulation Research·Jeanne James, Jeffrey Robbins
Jul 5, 2006·Journal of Muscle Research and Cell Motility·Briana K DenneheyKenneth S Krauter
May 20, 2003·The Journal of Biological Chemistry·Marian B MeyersGlenn I Fishman
May 20, 2003·The Journal of Biological Chemistry·Carmen C SucharovLeslie Leinwand
Dec 31, 1997·The American Journal of Physiology·J James, J Robbins
Nov 26, 2013·American Journal of Physiology. Heart and Circulatory Physiology·Lucia SantacruzDanny O Jacobs
Jan 22, 1998·The American Journal of Physiology·T CollinsE C Hardeman
Feb 20, 2007·American Journal of Physiology. Heart and Circulatory Physiology·Julia GigerFadia Haddad
Feb 18, 2021·Circulation·Priya UmapathiMark E Anderson
Jul 22, 2018·Journal of Molecular and Cellular Cardiology·Deborah U FrankJeffrey J Saucerman
Apr 12, 2000·Circulation·J JamesJ Robbins

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