An in vivo evaluation of microbial diversity before and after the photo-activated disinfection in primary endodontic infections: Traditional phenotypic and molecular approaches

Photodiagnosis and Photodynamic Therapy
Maryam Pourhajibagher, Abbas Bahador

Abstract

It is essential to identify the root canal microbial diversity and count. This is due to the polymicrobial nature of the primary endodontic infection that is associated with the microbial diversity and increased resistance to the antimicrobial agents. Photo-activated disinfection (PAD), also known as antimicrobial photodynamic therapy, is a new promising non-antibiotic approach, studied to prevent microbial resistance and treatment failure. In this study, we investigated the effect of PAD on reduction of microbial diversity and count, related with primary endodontic infections. Microbial specimens were collected before PAD from patients infected with the primary endodontic infection. PAD with toluidine blue O (TBO), in combination with diode laser, was performed on infected root canals. Resampling was carried out on the root canal after PAD, and microorganisms were identified by classical microbiological tests using biochemical and analytical profile index (API ® 20A) assays and nucleic acid approaches. From the 36 subjects studied before TBO-PAD, 187 cultivable isolates from 14 different genera and 19 various microbial species were retrieved. Of the bacterial isolates, 45.4% were strict anaerobes including Veillonella parvula,...Continue Reading

Citations

Mar 19, 2020·Journal of Clinical Medicine·Mario DioguardiLorenzo Lo Muzio
Dec 15, 2018·International Endodontic Journal·G PlotinoM Mercade
Feb 13, 2020·Journal of Clinical Medicine·Mario DioguardiGiuseppe Troiano
Dec 15, 2020·Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology·Antonio RomanoAlberta Lucchese
Mar 9, 2021·Restorative Dentistry & Endodontics·Amelia Wan Tin CheungGary Shun Pan Cheung
Jul 3, 2021·Biofouling·Vanessa Rodrigues Dos SantosCristiane Duque
Dec 23, 2021·Photobiomodulation, Photomedicine, and Laser Surgery·Ali Azhar Dawasaz

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