An in vivo internal deletion in the N-terminus region of Arabidopsis cystathionine gamma-synthase results in CGS expression that is insensitive to methionine

The Plant Journal : for Cell and Molecular Biology
Yael HachamRachel Amir

Abstract

Cystathionine gamma-synthase (CGS), the first enzyme of methionine biosynthesis in higher plants, plays an important role in the biosynthesis pathway and in regulating methionine metabolism in plant cells. In response to methionine, the expression of this enzyme is regulated via amino acid sequences located in its N-terminal. Here, using reverse transcription PCR and ribonuclease protection analysis, we demonstrate that, in addition to the full-length CGS transcript, a deleted form exists in Arabidopsis. The deleted transcript of CGS that lacks 90 or 87 nt located internally in the regulatory N-terminal region of CGS maintains the reading frame of the protein. Its association with polyribosomes indicates that this deleted form of CGS is translated. In order to study the function of this deleted form of CGS, we overexpressed it in transgenic tobacco plants. We found that the transgenic plants engineered to express only the deleted form of CGS accumulated methionine to a much higher level than those that expressed the full-length CGS. Furthermore, in vitro feeding experiments revealed that the deleted form of CGS is not subject to feedback regulation by methionine, as reported for the full-length transcript. Therefore, although m...Continue Reading

References

Jul 14, 1995·The Journal of Biological Chemistry·J F MaK Nomoto
Jun 24, 1998·Proceedings of the National Academy of Sciences of the United States of America·S RavanelR Douce
Jun 24, 2003·Trends in Plant Science·Holger Hesse, Rainer Hoefgen
Aug 23, 2003·Proceedings of the National Academy of Sciences of the United States of America·Yukako ChibaSatoshi Naito
Jul 6, 2004·Journal of Experimental Botany·Holger HesseRainer Hoefgen
Jun 1, 1990·Plant Physiology·S H Mudd, A H Datko

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Citations

Aug 13, 2014·Journal of Experimental Botany·Réjane Pratelli, Guillaume Pilot
Jan 7, 2016·Annual Review of Plant Biology·Gad GaliliAlisdair R Fernie
Jul 10, 2007·The Plant Journal : for Cell and Molecular Biology·Yael HachamRachel Amir
Jan 1, 2009·The Arabidopsis Book·Georg Jander, Vijay Joshi
Nov 30, 2019·The Plant Journal : for Cell and Molecular Biology·Anner CohenRachel Amir

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