PMID: 7088003Feb 22, 1982Paper

An in vivo method for the detection of somatic mutations at the cellular level in mice

Mutation Research
A G Searle, D A Stephenson


This new method for detection of somatic mutations is based on the fact that recessive mutations at the dilute (d) and leaden (ln) loci change the shape of pigment-cells in the hair follicles from their normal highly dendritic appearance (nucleofugal) to a rounded-up almost non-dendritic form (nucleopetal). Gene action is autonomous and individual mutant melanocytes can be recognised in whole mounts of dorsal skin made after the post-natal onset of melanogenesis. Foetal heterozygotes for d and ln were treated with X-rays (0-200 rad) or procarbazine (0-300 mg/kg body mass by maternal i.p. injection) 17 days after detection of a vaginal plug; whole mounts of dorsal skin (fixed 3 days after birth) were microscopically scanned for mutant melanocytes in the hair follicles.About half a million follicular melanocytes were present in the 2 mounts made per mouse. Mutant cells were mainly in the form of small intrafollicular clones; the few mount containing large interfollicular clones were excluded from the analysis. For both treatments, dose-response relationships showed good fits to both a quadratic and a power-law curve. Equations for the latter were: For X-rays, y = 3.2 x 10(-5) + 3.12 x 10(-9) D2.07; for procarbazine, y = 3.6 x 10(...Continue Reading


Feb 1, 1979·Mutation Research·U H Ehling, A Neuhäuser
Jan 1, 1974·Annual Review of Genetics·B Mintz
Jun 1, 1965·Developmental Biology·T C Mayer

Related Concepts

Dose-Response Relationship, Drug
Downy Hair
Mice, Mutant Strains
Mutagenicity Tests

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