An in vivo model of prostate carcinoma growth and invasion in bone

Cell and Tissue Research
Jane L FisherGail P Risbridger

Abstract

Prostatic carcinoma affects 1 in 11 men and targets bone with sclerotic metastases. The study of prostate carcinoma growth in bone has been hampered by the lack of suitable animal models. We have developed an in vivo model of prostate carcinoma growth in bone by inoculating three human prostate carcinoma cell lines (PC-3, DU-145, and LNCaP) into the tibia of congenitally athymic mice. Developing tumors were analyzed by radiographic, histologic, immunohistochemical, and in situ hybridization examination. Seven of the nine PC-3 inoculated mice and all (9/9) of the DU-145 inoculated mice developed tumors in the injected limb. In contrast, inoculation with LNCaP cells failed to produce tumors (0/9). Radiologically, the tumors had a mixed sclerotic/lytic appearance with extracortical extension. All the PC-3 tumors invaded the bone marrow cavity, cortical bone, and surrounding soft tissue. The DU-145 tumors were confined to the bone marrow cavity in 7/9 animals. CK18 and Ki67 localization identified the human tumor cells and their proliferative activity, respectively. The PC-3- and DU-145-induced tibial tumors expressed alpha(1)I procollagen and osteopontin mRNA, to varying degrees. All the tumors demonstrated an up-regulation of ost...Continue Reading

Citations

Dec 15, 2012·Acta neurochirurgica·Fateme SalehiGelareh Zadeh
May 8, 2009·Clinical & Experimental Metastasis·Ronald R GomesRobert A Sikes
Feb 9, 2012·Molecular Imaging and Biology : MIB : the Official Publication of the Academy of Molecular Imaging·Christopher T WinkelmannTimothy J Hoffman
Dec 3, 2010·The Journal of Biological Chemistry·Julie M QuachT John Martin
Nov 24, 2011·Prostate Cancer·Kenneth C Valkenburg, Bart O Williams
Apr 13, 2013·Endocrine-related Cancer·Xinyu WuZoran Culig
Oct 13, 2006·Archives of Histology and Cytology·Mitsuru AsakaMasaki Shimono
Oct 21, 2004·Cancer·David G BostwickBarry Timms
Mar 22, 2012·Contrast Media & Molecular Imaging·Wu QinghuaJiang Jianwei
Aug 27, 2014·International Journal of Cancer. Journal International Du Cancer·Kimberley J ReevesNicola J Brown
May 14, 2005·Cancer Letters·Hitoshi InoueAkihiko Okuyama
Sep 29, 2004·Cytokine & Growth Factor Reviews·Emma M A BallGail P Risbridger
May 17, 2005·Cancer Cell·Conor C LynchMitsuru Futakuchi
Nov 6, 2003·Clinical Orthopaedics and Related Research·Valentine B AndelaRegis J O'Keefe
Sep 9, 2017·Disease Models & Mechanisms·Laura Gómez-CuadradoValerie G Brunton
Nov 28, 2007·Journal of Biomolecular Screening·Richard HerrmannStig Linder
May 18, 2018·Nature Reviews. Urology·Richard B BerishHon S Leong
Jan 5, 2019·F1000Research·David HillBill Chaudhry
Mar 21, 2007·Cancer Epidemiology, Biomarkers & Prevention : a Publication of the American Association for Cancer Research, Cosponsored by the American Society of Preventive Oncology·Katri S SelanderArja Jukkola-Vuorinen
Feb 4, 2009·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Yong LiPamela J Russell

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