An in vivo model of reduced nucleus pulposus glycosaminoglycan content in the rat lumbar intervertebral disc

Spine
John I BoxbergerDawn M Elliott

Abstract

An in vivo model resembling early stage disc degeneration in the rat lumbar spine. Simulate the reduced glycosaminoglycan content and altered mechanics observed in intervertebral disc degeneration using a controlled injection of chondroitinase ABC (ChABC). Nucleus glycosaminoglycan reduction occurs early during disc degeneration; however, mechanisms through which degeneration progresses from this state are unknown. Animal models simulating this condition are essential for understanding disease progression and for development of therapies aimed at early intervention. ChABC was injected into the nucleus pulposus, and discs were evaluated via micro-CT, mechanical testing, biochemical assays, and histology 4 and 12 weeks after injection. At 4 weeks, reductions in nucleus glycosaminoglycan level by 43%, average height by 12%, neutral zone modulus by 40%, and increases in range of motion by 40%, and creep strain by 25% were found. Neutral zone modulus and range of motion were correlated with nucleus glycosaminoglycan. At 12 weeks, recovery of some mechanical function was detected as range of motion and creep returned to control levels; however, this was not attributed to glycosaminoglycan restoration, because mechanics were no longer...Continue Reading

References

Jan 1, 1987·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·R H PearceM E Adams
Jan 1, 1987·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·T S KellerT H Hansson
Nov 1, 1967·Clinica Chimica Acta; International Journal of Clinical Chemistry·H Stegemann, K Stalder
Mar 1, 1997·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·J C IatridisV C Mow
Jul 31, 1998·European Spine Journal : Official Publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society·U BerlemannR J Moore
Aug 15, 1998·Spine·H K Pokharna, F M Phillips
Jun 20, 2001·The Journal of Veterinary Medical Science·K YamadaM Sato
Apr 30, 2002·Spine·Mark D BrownAnneliese D Heiner
Nov 21, 2002·Biochemical Society Transactions·P J RoughleyJ Antoniou
Jan 1, 2003·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Jason P NorcrossLaurence E Dahners
Jun 5, 2004·Lab Animal·Marc-Antoine RousseauJeffrey C Lotz
Dec 21, 2004·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Joseph J Sarver, Dawn M Elliott
Feb 22, 2005·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·Kayvan R KeshariJohn Kurhanewicz
Feb 17, 2006·Annals of Biomedical Engineering·Wade JohannessenDawn M Elliott
Apr 13, 2006·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Christian W A PfirrmannNorbert Boos
May 23, 2006·Biomechanics and Modeling in Mechanobiology·C S YerramalliD M Elliott
Jul 26, 2006·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·John I BoxbergerDawn M Elliott
Feb 3, 2007·Spine·Grace D O'ConnellDawn M Elliott

❮ Previous
Next ❯

Citations

Nov 14, 2008·European Spine Journal : Official Publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society·Koichi Masuda
Jan 16, 2014·HSS Journal : the Musculoskeletal Journal of Hospital for Special Surgery·Sheela R DamleMatthew E Cunningham
Oct 25, 2011·Arthritis Research & Therapy·Ravi K PonnappanMakarand V Risbud
Dec 3, 2010·Disease Models & Mechanisms·Lachlan J SmithDawn M Elliott
Jun 17, 2010·Clinical Orthopaedics and Related Research·Matthew E CunninghamChisa Hidaka
Sep 1, 2009·Cellular and Molecular Bioengineering·James C IatridisC L Korecki
Feb 6, 2016·Veterinary and Comparative Orthopaedics and Traumatology : V.C.O.T·Fabienne NeveuJean-François Nisolle
Jan 29, 2013·The Spine Journal : Official Journal of the North American Spine Society·Samantha C W ChanBenjamin Gantenbein-Ritter
Sep 29, 2011·The Orthopedic Clinics of North America·Won C Bae, Koichi Masuda
May 23, 2008·The Spine Journal : Official Journal of the North American Spine Society·Alejandro A Espinoza OríasDawn M Elliott
Jun 3, 2015·Tissue Engineering. Part C, Methods·Gianluca VadalàVincenzo Denaro
Dec 21, 2012·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Suzanne E L DetigerTheo H Smit
May 13, 2014·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·Anna M WangEd X Wu
Feb 16, 2015·European Spine Journal : Official Publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society·Suzanne E L DetigerRoel J W Hoogendoorn
Jan 1, 2012·Stem Cells International·Umile Giuseppe LongoVincenzo Denaro
Aug 30, 2016·Osteoarthritis and Cartilage·S E GullbrandL J Smith
Nov 27, 2015·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Carla CunhaMário A Barbosa
Aug 3, 2017·Spine·Renato H S UetaFlávio Faloppa
Jan 10, 2018·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Michael D NewtonTristan Maerz
Nov 23, 2018·Science Translational Medicine·Sarah E GullbrandRobert L Mauck
Apr 5, 2013·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·John T MartinDawn M Elliott
Jul 3, 2020·JOR Spine·Juliane D GlaeserDmitriy Sheyn
Jul 23, 2020·Scientific Reports·Erica K WagnerAmbika G Bajpayee
Aug 11, 2020·Journal of Orthopaedic Surgery and Research·ZhiCheng ZhangFang Li
Jul 27, 2021·Journal of Investigative Surgery : the Official Journal of the Academy of Surgical Research·Yidian WangXuewen Kang

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.