An in vivo model to assess magnesium alloys and their biological effect on human bone marrow stromal cells

Acta Biomaterialia
Sayuri YoshizawaCharles Sfeir

Abstract

Magnesium (Mg) alloys have many unique qualities which make them ideal candidates for bone fixation devices, including biocompatibility and degradation in vivo. Despite a rise in Mg alloy production and research, there remains no standardized system to assess their degradation or biological effect on human stem cells in vivo. In this study, we developed a novel in vivo model to assess Mg alloys for craniofacial and orthopedic applications. Our model consists of a collagen sponge seeded with human bone marrow stromal cells (hBMSCs) around a central Mg alloy rod. These scaffolds were implanted subcutaneously in mice and analyzed after eight weeks. Alloy degradation and biological effect were determined by microcomputed tomography (microCT), histological staining, and immunohistochemistry (IHC). MicroCT showed greater volume loss for pure Mg compared to AZ31 after eight weeks in vivo. Histological analysis showed that hBMSCs were retained around the Mg implants after 8 weeks. Furthermore, immunohistochemistry showed the expression of dentin matrix protein 1 and osteopontin around both pure Mg and AZ31 with implanted hBMSCs. In addition, histological sections showed a thin mineral layer around all degrading alloys at the alloy-tiss...Continue Reading

References

Sep 1, 1977·Journal of Biomedical Materials Research·M Chvapil
Nov 1, 1992·The Journal of Bone and Joint Surgery. British Volume·R K Fraser, W G Cole
Jan 1, 1988·Trends in Biochemical Sciences·T L Macdonald, R B Martin
Jan 1, 1995·Journal of Applied Biomaterials : an Official Journal of the Society for Biomaterials·D A Puleo, W W Huh
Mar 5, 1998·The Journal of Bone and Joint Surgery. American Volume·J J JacobsR M Urban
Aug 15, 1998·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·W Friess
Nov 21, 2000·Biotechnology and Bioengineering·M H MankaniP G Robey
Oct 24, 2002·Der Chirurg; Zeitschrift für alle Gebiete der operativen Medizen·L Claes, A Ignatius
Nov 19, 2003·Advanced Drug Delivery Reviews·M GeigerW Friess
Aug 27, 2005·Biomaterials·Frank WitteHenning Windhagen
Sep 6, 2005·Bone·Lorenz MeinelDavid Kaplan
Oct 26, 2005·Biomaterials·Mark P StaigerGeorge Dias
Feb 10, 2006·The Journal of the American Academy of Orthopaedic Surgeons·Matthew L BusamWilliam T Obremskey
Nov 24, 2006·Advances in Experimental Medicine and Biology·Pamela HabibovicClemens van Blitterswijk
Mar 29, 2007·Journal of Biomedical Materials Research. Part a·F WitteE Willbold
Mar 29, 2007·Journal of Biomedical Materials Research. Part a·F WitteE Willbold
Jul 4, 2007·Journal of Materials Science. Materials in Medicine·Alexis PietakMark P Staiger
May 22, 2008·Journal of Tissue Engineering and Regenerative Medicine·Manabu AkahaneYoshinori Takakura
May 27, 2008·BMC Musculoskeletal Disorders·Beate HansonDirk Stengel
Nov 13, 2008·Biomaterials·Xuenan GuTingfei Xi
Oct 14, 2009·Acta Biomaterialia·F WitteF Beckmann
Nov 5, 2011·Regenerative Medicine·Albert D DonnenbergWp Andrew Lee
Feb 15, 2012·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Jemimah WalkerGeorge J Dias
Dec 4, 2013·Acta Biomaterialia·Patrick K BowenJeremy Goldman
Jan 5, 2014·Acta Biomaterialia·Sarah E HendersonAlejandro J Almarza
Jan 25, 2014·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Jemimah WalkerGeorge J Dias
Jan 13, 2015·Journal of Oral and Maxillofacial Surgery : Official Journal of the American Association of Oral and Maxillofacial Surgeons·Amy ChayaCharles Sfeir
Apr 10, 2015·Journal of Biomedical Materials Research. Part a·Mohamed Ruslan AbdullahMat Uzir Wahit

❮ Previous
Next ❯

Citations

Feb 7, 2016·Biomaterials·Daniel M VasconcelosMário A Barbosa
May 7, 2019·International Journal of Molecular Sciences·Chin-Chean WongChih-Hwa Chen
Mar 15, 2018·International Journal of Molecular Sciences·Kristina GlenskeMike Barbeck
Oct 1, 2020·International Journal of Molecular Sciences·Fabiana da Silva Lima, Ricardo Ambrósio Fock
Jul 11, 2016·ACS Biomaterials Science & Engineering·Nicole OstrowskiPrashant N Kumta

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.