An in vivo requirement for the mediator subunit med14 in the maintenance of stem cell populations

Stem Cell Reports
Jeffrey T A BurrowsIan C Scott

Abstract

The Mediator complex has recently been shown to be a key player in the maintenance of embryonic and induced pluripotent stem cells. However, the in vivo consequences of loss of many Mediator subunits are unknown. We identified med14 as the gene affected in the zebrafish logelei (log) mutant, which displayed a morphological arrest by 2 days of development. Surprisingly, microarray analysis showed that transcription was not broadly affected in log mutants. Indeed, log cells transplanted into a wild-type environment were able to survive into adulthood. In planarians, RNAi knockdown demonstrated a requirement for med14 and many other Mediator components in adult stem cell maintenance and regeneration. Multiple stem/progenitor cell populations were observed to be reduced or absent in zebrafish med14 mutant embryos. Taken together, our results show a critical, evolutionarily conserved, in vivo function for Med14 (and Mediator) in stem cell maintenance, distinct from a general role in transcription.

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Citations

Nov 16, 2016·International Journal of Molecular Sciences·Jie YangFang Zheng
Nov 11, 2015·BMC Developmental Biology·Xin LouIan C Scott

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Methods Mentioned

BETA
transgenic
PCR
amputation
gene trap
transgenics
in vitro transcription

Software Mentioned

Genespring
FlowJo

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