An in vivo selection system for homing endonuclease activity

Nucleic Acids Research
Mathias GruenDavid R Liu

Abstract

Homing endonucleases are enzymes that catalyze the highly sequence-specific cleavage of DNA. We have developed an in vivo selection in Escherichia coli that links cell survival with homing endonuclease-mediated DNA cleavage activity and sequence specificity. Using this selection, wild-type and mutant variants of three homing endonucleases were characterized without requiring protein purification and in vitro analysis. This selection system may facilitate the study of sequence-specific DNA cleaving enzymes, and selections based on this work may enable the evolution of homing endonucleases with novel activities or specificities.

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Citations

Nov 7, 2002·Molecular Cell·Brett S ChevalierBarry L Stoddard
Jul 21, 2010·Proceedings of the National Academy of Sciences of the United States of America·Taegun KwonDavid L Herrin
Feb 4, 2006·Nucleic Acids Research·James C SamuelsonShuang-yong Xu
Oct 20, 2007·Nucleic Acids Research·Jennifer H EastbergBarry L Stoddard
Jul 13, 2004·Nucleic Acids Research·Nobuhide DoiHiroshi Yanagawa
Oct 11, 2005·Nucleic Acids Research·Zhilei Chen, Huimin Zhao
Jan 30, 2009·Protein Engineering, Design & Selection : PEDS·Zhilei ChenHuimin Zhao
Nov 17, 2009·Cellular and Molecular Life Sciences : CMLS·Maria J MarcaidaGuillermo Montoya
Aug 18, 2004·Journal of Molecular Biology·Django SussmanLenny M Seligman
Jul 29, 2006·Journal of Molecular Biology·George H SilvaAlfred Pingoud
May 12, 2011·Chembiochem : a European Journal of Chemical Biology·Alfred Pingoud, Wolfgang Wende
May 3, 2018·Nucleic Acids Research·Adam J BogdanoveBarry L Stoddard
Feb 16, 2006·Journal of the American Chemical Society·Jeffrey B DoyonDavid R Liu

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