An in vivo stable isotope-based approach for assessment of absorbed amino acids from individual feed ingredients within complete diets

Journal of Dairy Science
K A EstesM D Hanigan

Abstract

Accurate assessment of the nutritional content of feed ingredients is required for precise diet formulation. Characterizing ingredients in terms of absorption and digestibility of individual AA is challenging, and this information often relies on indirect methods. The purpose of this research was to evaluate an in vivo stable isotope-based method of determining plasma entry rates of individual AA from feather meal (FM), blood meal (BM), and a rumen-protected AA (RPMet). Abomasal infusions of unprotected Ile, Leu, Met, and sodium caseinate were used as control treatments to assess technique reliability and accuracy. Isotopic enrichment of plasma AA in response to a 2-h constant jugular infusion of a mixture of 13C labeled AA was measured and modeled using a dynamic 4-pool model, which was fitted to each AA by infusion to derive diet entry rates. The resulting entry rate matrix was used to derive plasma entry rates of individual AA from each ingredient by regression. The mean of plasma AA entry for abomasally infused Ile, Leu, and Met was 93.4 ± 7.35% of that infused, indicating that 6.6% was used by splanchnic tissues during first pass. The mean of the plasma essential AA entry for abomasally infused casein was 86.7 ± 4.81% of t...Continue Reading

References

Sep 1, 1990·Journal of Animal Science·F K GoedekenM H Sindt
Sep 1, 1990·Journal of Animal Science·F K GoedekenR A Britton
Sep 1, 1965·Canadian Journal of Biochemistry·A E Harper
Apr 1, 1972·The Journal of Nutrition·R R OltjenR L Wilson
May 1, 1995·Journal of Animal Science·S Calsamiglia, M D Stern
May 1, 1993·Journal of Animal Science·S J AinslieM C Barry
Aug 1, 1997·Journal of Animal Science·M D SternS Calsamiglia
Jan 7, 1998·Journal of Animal Science·M J KlemesrudD W Herold
May 22, 1998·Journal of Dairy Science·S M Rutherfurd, P J Moughan
Feb 22, 2000·Journal of Animal Science·M J KlemesrudA J Lewis
Apr 20, 2004·Journal of Theoretical Biology·M D HaniganJ France
May 1, 2009·The Journal of Nutrition·Mark D HaniganBrian J Bequette
Dec 24, 2011·Journal of Dairy Science·H LapierreG E Lobley
Feb 1, 2008·Animal : an International Journal of Animal Bioscience·S I Borucki CastroR Berthiaume

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