An in vivo study of free radicals generated in murine skin by protoporphyrin IX and visible light

Photochemistry and Photobiology
Kozo NakaiColin F Chignell

Abstract

Lipids extracted from the skin of C57BL/6J mice injected subcutaneously with alpha-(4-pyridyl-1-oxide)-N-tert-butylnitrone (POBN) and exposed to topical protoporphyrin IX (PPIX) and visible light had significantly higher levels of POBN spin adducts compared with dark PPIX exposed or vehicle-treated controls. Computer analysis of the POBN adduct electron paramagnetic (spin) resonance (EPR) spectra indicated that two radical species were present in each extract, one of which was a lipid-derived carbon-centered adduct (1, a(N) = 14.8 G and a(H) = 2.6 G), whereas the other (2, a(N) = 13.8 G and a(H) = 1.8 G) was probably oxygen centered. Adduct 2 was present in greater proportion in lipids extracted from PPIX/light-exposed mice compared with dark or vehicle-treated controls. These findings suggest that PPIX/light generates free radicals in mouse skin, thus providing a radical mechanism for PPIX-induced photosensitivity. Our approach may be useful for the detection of free radicals generated by other skin photosensitizers and may also provide a means for testing putative skin-protecting agents.

References

Jan 1, 1991·Current Problems in Dermatology·H Baart de la FailleH van Weelden
Dec 15, 1986·Biochemical and Biophysical Research Communications·C MottleyR P Mason
Jun 1, 1994·Journal of Magnetic Resonance. Series B·D R Duling
May 22, 2002·Drug Safety : an International Journal of Medical Toxicology and Drug Experience·Douglas E Moore
Sep 20, 2002·Skin Pharmacology and Applied Skin Physiology·T HerrlingN Groth
Nov 1, 2002·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Keizo SatoRonald P Mason
Aug 27, 2005·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Hiromu SakuraiMariko Shigemoto
Dec 1, 2004·Photodiagnosis and Photodynamic Therapy·Ana P CastanoMichael R Hamblin

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BETA
electron paramagnetic

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WINSIM

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