An Independent Evaluation of the CryoXtract Instruments' CXT350 Frozen Sample Aliquotter Using Tissue and Fecal Biospecimens

Biopreservation and Biobanking
William MathiesonFay Betsou

Abstract

The ability to take targeted multiple cores from a single frozen biospecimen would enable several research projects to be fueled from one biospecimen, a small piece of tissue to be quality-control tested, and for pathologically-discrete areas of a biospecimen (e.g., tumor, stromal, and normal tissue) to be selectively sampled for comparative analyses. CryoXtract Instruments' CXT350 Frozen Sample Aliquotter can potentially achieve this by producing multiple cores from one cryopreserved biospecimen without thawing either the parent biospecimen or its daughter cores. It therefore has the potential to add significant value to a tissue banking workflow. We have evaluated its performance while using 614 cores from fecal, liver, kidney, lung, heart, and colon biospecimens. Coring densities of up to five complete and four fragmentary cores per cm(3) are achievable using 3 mm coring probes. Median core weights for tissue were 14.1-17.2 mg (depending on tissue type) and cores ≤325 mg could be taken from fecal biospecimens (depending on the fill-depth of the tube). The coefficient of variation for multiple cores taken from a fecal biospecimen was 11.7%. Between-sample contamination did not occur. RNA Integrity numbers and qRT-PCR analysis...Continue Reading

References

Jun 16, 2011·Journal of the National Cancer Institute. Monographs·Holly A MassettCarolyn Compton
Jan 10, 2012·Annual Review of Immunology·Kenya Honda, Dan R Littman
Feb 7, 2013·Environmental Health Perspectives·Dennie G A J HebelsUNKNOWN EnviroGenomarkers Project Consortium
May 23, 2014·Biopreservation and Biobanking·Helena Judge Ellis, Deborah S Venturini

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Citations

Jan 31, 2020·Biopreservation and Biobanking·Lorie Neuberger-CastilloFay Betsou
Sep 21, 2017·Biopreservation and Biobanking·Xiaoli JiJunmei Zhou

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Methods Mentioned

BETA
electrophoresis
PCR

Software Mentioned

SDS

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