An indirect competitive immunoassay for insulin autoantibodies based on surface plasmon resonance

Biosensors & Bioelectronics
Jenny CarlssonBo Liedberg

Abstract

We have developed a sensitive and specific method based on surface plasmon resonance (SPR) for detection of insulin autoantibodies (IAA) in serum samples from individuals at high risk of developing type 1 diabetes (T1D). When measuring trace molecules in undiluted sera with label-free techniques like SPR, non-specific adsorption of matrix proteins to the sensor surface is often a problem, since it causes a signal that masks the analyte response. The developed method is an indirect competitive immunoassay designed to overcome these problems. Today, IAA is mainly measured in radio immunoassays (RIAs), which are time consuming and require radioactively labeled antigen. With our SPR-based immunoassay the overall assay time is reduced by a factor of >100 (4 days to 50min), while sensitivity is maintained at a level comparable to that offered by RIA.

References

Mar 5, 2003·Diabetes Technology & Therapeutics·William E WinterDesmond Schatz
Apr 30, 2003·Diabetes·Polly J BingleyPatricia W Mueller
Apr 14, 2005·Best Practice & Research. Clinical Endocrinology & Metabolism·Alberto Falorni, Annalisa Brozzetti
Nov 25, 2005·Diabetes·Peter AchenbachAnette-G Ziegler
Nov 25, 2005·Diabetes·Catherine PihokerAke Lernmark
Jan 9, 2007·Biomacromolecules·Andréas LarssonBo Liedberg

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Citations

Jun 22, 2010·Analytical and Bioanalytical Chemistry·Bastian E RappKerstin Länge
Feb 26, 2014·Analytical and Bioanalytical Chemistry·Stefano Mariani, Maria Minunni
Nov 10, 2011·Diabetes/metabolism Research and Reviews·Patrick MarcusWilliam Hagopian
Dec 18, 2009·Journal of Molecular Recognition : JMR·Rebecca L Rich, David G Myszka
Sep 22, 2009·The Analyst·Asta Kausaite-MinkstimieneArunas Ramanavicius
Aug 12, 2021·Analytical Methods : Advancing Methods and Applications·Yang XiaoJinhang Gao
Jul 20, 2017·ACS Sensors·Jean-Francois Masson

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