PMID: 9186940May 1, 1997Paper

An indirect ELISA for the detection of antibodies against porcine reproductive and respiratory syndrome virus using recombinant nucleocapsid protein as antigen

Journal of Virological Methods
H DenacMartin A Hofmann

Abstract

An indirect enzyme-linked immunosorbent assay termed rnPRRS ELISA using baculovirus-expressed and affinity-purified viral nucleocapsid protein (rNC) antigen was developed for detecting antibodies against porcine reproductive and respiratory syndrome virus (PRRSV) in swine sera. Sera (1395) originating from different European countries were used for the validation of this assay. The rnPRRS ELISA was capable of detecting antibodies in all sera known to contain anti-PRRSV antibodies, resulting in 100% sensitivity. The specificity was 95.8%. The rnPRRS ELISA was more sensitive compared to the most widely used tests for the diagnosis of porcine reproductive and respiratory syndrome (PRRS) (i) a commercially available ELISA; (ii) the indirect immunofluorescence assay (IIFA); and (iii) the immunoperoxidase monolayer assay (IPMA). The main advantage of the rnPRRS ELISA compared to an ELISA using whole virus antigen is the use of a single immunogenic protein instead of infectious PRRSV. The rnPRRS ELISA is suitable for routine diagnosis of PRRS and also for epidemiological surveys since it allows highly reliable testing of a large number of sera within a short period of time.

References

Jan 10, 1995·Virology·J J MeulenbergG Wensvoort

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Citations

Mar 19, 1999·Preventive Veterinary Medicine·L Audigé, S Beckett
Sep 17, 2010·Expert Review of Molecular Diagnostics·Vinayagamurthy BalamuruganRaj Kumar Singh
Nov 30, 2005·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Y S LyooJ H Sur
Aug 25, 2004·Comparative Immunology, Microbiology and Infectious Diseases·H Takahashi-OmoeT Inada
Aug 21, 2015·The Journal of Veterinary Medical Science·Byoung-Joo SeoWon-Il Kim
Jan 30, 1999·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·P GoninS Dea
Nov 5, 1998·Clinical and Diagnostic Laboratory Immunology·S K WoottonD Yoo

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