PMID: 1206224Dec 1, 1975Paper

An indirect radioimmunoassay for mouse casein using 125I-labeled antigen

Journal of Immunological Methods
P M TerryM R Banerjee

Abstract

A new indirect radioimmunoassay was developed for detection of casein in mouse milk and in mammary tissue extract. Preincubation of rabbit gamma globulin to mouse milk casein (Ca2+-rennin precipitate) with unlabeled casein, milk, extracts of mammary tissues of late pregnancy and lactation, virtually blocked subsequent binding of 125I-labeled mouse milk casein to the antibody. Preincubation with mouse serum, bovine serum albumin, rennin, extracts of liver or immature mammary tissue had little effect on [125I]casein binding to the antibody. The inability of [125I]casein to bind to the antibody after preincubation with protein samples, which are likely to contain casein, is indicative of a specific antigen-antibody reaction. The assay is capable of detecting 0.2 mug casein, 1 mug milk proteins and 10 mug lactating mammary tissue extract. The application of the assay was also demonstrated using organ culture of the entire mammary gland. The glands treated with the lactogenic hormones, insulin + prolactin + cortisol, showed a saturation level of antibody-antigen reaction, indicating hormonal induction of casein; whereas, no reaction was observed with the non-treated gland.

References

Sep 1, 1974·Comparative Biochemistry and Physiology. A, Comparative Physiology·M K Feldman
Nov 1, 1974·Journal of the National Cancer Institute·M R BanerjeeL L Washburn
Dec 1, 1970·Comparative Biochemistry and Physiology·M K Feldman, R L Ceriani
Dec 16, 1965·Biochimica Et Biophysica Acta·R W TurkingtonY J Topper

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Citations

Jan 1, 1978·In Vitro·M R BanerjeeR Ganguly
Jan 1, 1977·Journal of Immunological Methods·J Enami, S Nandi
Jan 1, 1987·Comparative Biochemistry and Physiology. A, Comparative Physiology·F Maher, K R Nicholas
Dec 1, 1977·Molecular and Cellular Endocrinology·P M TerryM R Banerjee
Oct 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·J T EmermanS Nandi
Jun 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·P M TerryR M Lui
Dec 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·R GangulyM R Banerjee
Oct 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·R GangulyM R Banerjee
Sep 1, 1977·Journal of Toxicology and Environmental Health·M R BanerjeeF K Lin
Dec 18, 2001·Developmental Dynamics : an Official Publication of the American Association of Anatomists·E Gallo-HendrikxZ X Xiao
Jun 15, 1981·International Journal of Cancer. Journal International Du Cancer·Q J TonelliS Sorof

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