An individually addressable suspended-drop electroporation system for high-throughput cell transfection

Lab on a Chip
Youchun XuWanli Xing

Abstract

High-efficiency transfection of genes, proteins, or drug compounds into cells without causing permanent damage is a prerequisite for many cell biology experiments. Here, we report a printed circuit board (PCB)-based electroporation device for high-throughput delivery of exogenous molecules into cells in an individually addressable manner. This device incorporates an array of 96 through-holes on the PCB with a pair of gold coated symmetric electrodes plated on the wall of each through-hole. A mixture of cell suspension and exogenous molecules is top-loaded, electroporated, and bottom-ejected with cell culture medium into the corresponding well of a 96-well plate placed under the PCB. One group of electrodes on the same side of the 96 through-holes were connected to eight top pads via connecting row wires plated on the top surface of the PCB and the other group of electrodes were connected in a column format to twelve bottom pads. Therefore, each pair of electrodes can be selectively energized, guaranteeing that appropriate electroporation parameters can be applied to different holes. We demonstrate the use of the present device to effectively introduce plasmid DNA and synthetic interfering RNA into cultured and primary cells wit...Continue Reading

References

Nov 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·P L FelgnerM Danielsen
Apr 1, 1995·Neuron·D L PettitR Malinow
May 3, 2001·Nature·J Ziauddin, D M Sabatini
Oct 4, 2003·Genome Research·Spyro MoussesOlli Kallioniemi
Apr 16, 2004·Proceedings of the National Academy of Sciences of the United States of America·Jose M SilvaGregory J Hannon
Mar 23, 2005·Nature Methods·Ben Lehner, Andrew G Fraser
May 28, 2005·Nature Genetics·Douglas B WheelerDavid M Sabatini
Aug 1, 2006·Biochemical and Biophysical Research Communications·S S ChungK S Park
Feb 5, 2008·Biosensors & Bioelectronics·Jeong Ah KimJun Keun Chang
Apr 15, 2008·Nature Methods·Emmanuel G Guignet, Tobias Meyer
Feb 2, 2010·Journal of Controlled Release : Official Journal of the Controlled Release Society·Tao GengChang Lu
May 12, 2011·PloS One·Pablo TurjanskiGuillermo Marshall
Jan 17, 2012·Lab on a Chip·Tilak JainEnrique Saez
Nov 28, 2012·Lab on a Chip·Nima JokilaaksoRashid Bashir

❮ Previous
Next ❯

Citations

Apr 30, 2016·International Journal of Heat and Mass Transfer·Yi LuPak Kin Wong
Jun 21, 2019·Electrophoresis·Jun Yuan ChanKostya Ken Ostrikov
Apr 9, 2015·Journal of Biomolecular Screening·Christin Luft, Robin Ketteler
Jun 27, 2015·The Journal of Experimental Biology·Gen ZhangPei-Yuan Qian

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

© 2021 Meta ULC. All rights reserved