An inducible expression vector for both fission and budding yeast

Gene
D PicardK R Yamamoto

Abstract

We have developed a vector system for inducible gene expression in both fission yeast (Schizosaccharomyces pombe) and budding yeast (Saccharomyces cerevisiae). The autonomously replicating expression vector contains multiple glucocorticoid response elements, rendering a linked promoter inducible 20-70-fold by glucocorticoid hormones in the presence of the mammalian glucocorticoid receptor. A polylinker with several unique cloning sites allows insertion of cDNAs of interest. Glucocorticoids are gratuitous signalling molecules in yeast, exerting little or no effect on the expression of genes other than those fused to the regulated promoter.

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Citations

Jul 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·K S ChristophersonP J Godowski
May 9, 1995·Proceedings of the National Academy of Sciences of the United States of America·A KralliK R Yamamoto
May 1, 1995·DNA and Cell Biology·Y Zhao, H B Lieberman
Nov 1, 1992·Molecular Biology of the Cell·M J Garabedian, K R Yamamoto
Jun 8, 2004·Journal of Experimental Botany·Wei Tang, Ronald J Newton
Jun 1, 1997·Annual Review of Plant Physiology and Plant Molecular Biology·C. Gatz
Apr 26, 2006·Proceedings of the National Academy of Sciences of the United States of America·Chris PhelpsDidier Picard
Mar 17, 2016·Microbial Cell Factories·Rosario VanellaEric Kübler
Jun 1, 1992·Yeast·M A RomanosJ J Clare
Feb 20, 1999·Journal of Molecular Biology·S RanaP K Chakraborti
Apr 9, 2004·Molecular Endocrinology·Gregory M NelsonDavid F Smith
Mar 21, 1998·FEMS Microbiology Letters·J W Liu, D Picard

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