PMID: 3755200Apr 1, 1986Paper

An inexpensive and interactive microcomputer system for codifying Golgi-impregnated neuronal morphology

Journal of Neuroscience Methods
Miguel Freire

Abstract

An interactive microcomputer system has been developed for the quantitative analysis of Golgi-impregnated neuronal morphology (GINM). The system uses commercially available hardware: a 48K RAM microcomputer (Apple II), a TV monitor (NEC), an optical microscope (Reichert Biovar) with a camera lucida and a specially developed wheel attached to the focus adjustment knob and to a digital planimeter (Tamaya), allowing one to obtain the value of the z-coordinate. The x- and y-coordinates are obtained using the movement of a point plotted by the computer on the TV monitor. This point is superimposed upon the GINM point to be codified employing the camera lucida. The x- and y-coordinates are acquired automatically while the variations of the z-coordinate are introduced by the computer keyboard. The three-dimensional coordinates of the selected GINM points and different codes are stored by the computer in a six-dimensional array. The software is written in BASIC. The results demonstrate that the digitization and quantitative analysis of GINM can be achieved with reasonably inexpensive equipment.

References

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Citations

Jul 1, 1987·Journal of Neuroscience Methods·M Freire
Sep 1, 1988·Journal of Neuroscience Methods·A Alvarez-Buylla, D S Vicario
Mar 1, 1990·Journal of Neuroscience Methods·M LowndesM G Stewart
Dec 1, 1990·Journal of Neuroscience Methods·M Freire
Jan 1, 1991·Journal of Neuroscience Methods·G M Ratto, C Usai
Jan 1, 1990·International Quarterly of Community Health Education·J AubelB Coulibaly
Jul 1, 1996·Journal of General Internal Medicine·P R Lichstein, G Young
Aug 26, 1998·The British Journal of Surgery·J Camilleri-Brennan, R J Steele

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