PMID: 3755201Apr 1, 1986Paper

An inexpensive microcomputer digital imaging system for densitometry: quantitative autoradiography of insulin receptors with 125I and LKB Ultrofilm

Journal of Neuroscience Methods
D G BaskinM K Graham

Abstract

This article describes a video digitizing system designed for measuring film optical density. The system, which is based on a 6-bit (64 gray level) digitizer, solid state video camera, and Apple II microcomputer, digitizes a rectangular area selected by the operator and converts the gray level values into preselected standard units. In order to develop autoradiographic standard curves for quantitative autoradiography with 125I-insulin, liver slices labeled with 125I-insulin and plastic sections containing known amounts of tritium were apposed to the same sheet of LKB Ultrofilm for exposures of 1-7 days. The results indicate that 3H plastic standards can be used to calibrate QAR of 125I-labeled ligands with LKB Ultrofilm. The Apple system was also used to measure binding of 125I-insulin to the external plexiform layer (EPL) in slices of the rat olfactory bulb. Results suggest that the EPL has two binding sites for insulin, a high affinity site with Kd = 1.0 X 10(-8) M and a low affinity site having a Kd = 1.4 X 10(-5) M.

References

May 1, 1985·Journal of Neuroscience Methods·D J Berck, T C Rainbow
Sep 1, 1980·Analytical Biochemistry·P J Munson, D Rodbard
Jan 1, 1982·Neuroscience and Biobehavioral Reviews·C R GallistelM Negin
Jun 1, 1984·Journal of Neuroscience Methods·P RammB J Frost
Nov 1, 1984·Journal of Neuroscience Methods·G M Alexander, R J Schwartzman
Mar 1, 1984·Journal of Neuroscience Methods·C A AltarJ F Marshall

❮ Previous
Next ❯

Citations

Apr 1, 1993·Physiology & Behavior·D G BaskinM W Schwartz
Jan 1, 1995·Brain Research. Brain Research Reviews·D N Ishii
Jun 1, 1988·Journal of Neuroscience Methods·J I ChocaH K Proudfit
Aug 1, 1988·Journal of Neuroscience Methods·A P Davenport, M D Hall
Jun 1, 1990·Journal of Neuroscience Methods·R ArtymyshynB B Wolfe
Mar 1, 1988·Trends in Neurosciences·D G BaskinD M Dorsa
Jan 1, 1991·Neuroscience and Biobehavioral Reviews·C R Plata-Salamán
Aug 1, 1998·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·M WatanabeM Shimada
Jan 1, 1990·International Journal of Radiation Applications and Instrumentation. Part B, Nuclear Medicine and Biology·U SchmidS Matzku
Jun 3, 2009·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Susanna FreudeMarkus Schubert
Oct 1, 1990·The American Journal of Physiology·D J KoerkerD G Baskin

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.