An Infant Mouse Model of Influenza Virus Transmission Demonstrates the Role of Virus-Specific Shedding, Humoral Immunity, and Sialidase Expression by Colonizing Streptococcus pneumoniae

MBio
Mila Brum OrtigozaJeffrey N Weiser

Abstract

The pandemic potential of influenza A viruses (IAV) depends on the infectivity of the host, transmissibility of the virus, and susceptibility of the recipient. While virus traits supporting IAV transmission have been studied in detail using ferret and guinea pig models, there is limited understanding of host traits determining transmissibility and susceptibility because current animal models of transmission are not sufficiently tractable. Although mice remain the primary model to study IAV immunity and pathogenesis, the efficiency of IAV transmission in adult mice has been inconsistent. Here we describe an infant mouse model that supports efficient transmission of IAV. We demonstrate that transmission in this model requires young age, close contact, shedding of virus particles from the upper respiratory tract (URT) of infected pups, the use of a transmissible virus strain, and a susceptible recipient. We characterize shedding as a marker of infectiousness that predicts the efficiency of transmission among different influenza virus strains. We also demonstrate that transmissibility and susceptibility to IAV can be inhibited by humoral immunity via maternal-infant transfer of IAV-specific immunoglobulins and modifications to the ...Continue Reading

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Citations

Nov 7, 2019·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Hannah M Rowe, Jason W Rosch
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Jun 3, 2021·Viruses·Thi-Quyen NguyenYoung-Ki Choi
May 20, 2021·MBio·Hannah M Rowe, Jason W Rosch
Aug 20, 2020·Nature Communications·Sima AsadiNicole M Bouvier

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Methods Mentioned

BETA
lavages
ELISA
lavage
enzyme-linked immunosorbent assay
reverse transcription-PCR
PCR

Software Mentioned

GraphPad Prism

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