An Infectious cDNA Clone of SARS-CoV-2

Cell Host & Microbe
Xuping XiePei-Yong Shi


The ongoing pandemic of COVID-19, caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), underscores the urgency to develop experimental systems for studying this virus and identifying countermeasures. We report a reverse genetic system for SARS-CoV-2. Seven complimentary DNA (cDNA) fragments spanning the SARS-CoV-2 genome were assembled into a full-genome cDNA. RNA transcribed from the full-genome cDNA was highly infectious after electroporation into cells, producing 2.9 × 106 plaque-forming unit (PFU)/mL of virus. Compared with a clinical isolate, the infectious-clone-derived SARS-CoV-2 (icSARS-CoV-2) exhibited similar plaque morphology, viral RNA profile, and replication kinetics. Additionally, icSARS-CoV-2 retained engineered molecular markers and did not acquire other mutations. We generated a stable mNeonGreen SARS-CoV-2 (icSARS-CoV-2-mNG) by introducing this reporter gene into ORF7 of the viral genome. icSARS-CoV-2-mNG was successfully used to evaluate the antiviral activities of interferon (IFN). Collectively, the reverse genetic system and reporter virus provide key reagents to study SARS-CoV-2 and develop countermeasures.


Jun 25, 2020·The Journal of General Virology·Severino Jefferson Ribeiro da SilvaLindomar Pena
Jul 30, 2020·PLoS Pathogens·Margarida Sa RiberoSébastien Nisole
Jul 24, 2020·Journal of Virology·Abigail VanderheidenMehul S Suthar
Aug 14, 2020·Nature·Mark J MulliganKathrin U Jansen
Sep 27, 2020·MBio·Chengjin YeLuis Martinez-Sobrido
Sep 30, 2020·The New England Journal of Medicine·Evan J AndersonUNKNOWN mRNA-1273 Study Group
Jul 17, 2020·Nature Nanotechnology·Matthew D ShinNicole F Steinmetz
Aug 15, 2020·Nature Communications·Antonio E MuruatoPei-Yong Shi
Jul 2, 2020·International Journal of Molecular Sciences·Alejandro LlanesRicardo Lleonart
Sep 25, 2020·Journal of Translational Medicine·Francesca CaccuriArnaldo Caruso
Sep 9, 2020·PLoS Biology·Nicole A P LiebermanAlexander L Greninger
Oct 14, 2020·Current Protocols in Microbiology·Kevin ChiemLuis Martinez-Sobrido
Oct 31, 2020·Wiener klinische Wochenschrift·Franz X Heinz, Karin Stiasny
Nov 5, 2020·Microorganisms·Ioana Roxana BordeaFrancesco Inchingolo
Nov 6, 2020·PloS One·Eric C RouchkaDonghoon Chung
Nov 21, 2020·Current Protocols in Immunology·Abigail VanderheidenMehul S Suthar
Nov 27, 2020·Proceedings of the National Academy of Sciences of the United States of America·Ruochen ZangSiyuan Ding
Oct 28, 2020·Nature·Jessica A PlantePei-Yong Shi
Dec 19, 2020·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Hongjie Xia, Pei-Yong Shi
Dec 29, 2020·Advanced Drug Delivery Reviews·Jee Young ChungYoung Jik Kwon
Nov 2, 2020·Diagnostic Microbiology and Infectious Disease·Xuping XiePing Ren
Dec 15, 2020·Journal of Molecular Biology·Takamitsu HattoriShohei Koide
Sep 28, 2020·Cell Reports·Hongjie XiaPei-Yong Shi
Jan 28, 2021·Scientific Reports·Tomohiro KotakiMasanori Kameoka
Oct 30, 2020·Nature Reviews. Microbiology·Philip V'kovskiVolker Thiel
Feb 13, 2021·AIMS Public Health·Srikanth UmakanthanMaryann Bukelo
Jan 7, 2021·Nature Structural & Molecular Biology·Robert J EdwardsPriyamvada Acharya

❮ Previous
Next ❯

Methods Mentioned

in vitro transcription
Fluorescence microscopy

Software Mentioned

GraphPad Prism
Adobe illustrator

Related Concepts

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.


Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.


Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

American Journal of Clinical Pathology
Ahmed BabikerJeannette Guarner
The New England Journal of Medicine
Xiaoxia LuChinese Pediatric Novel Coronavirus Study Team
Journal of Medical Virology
Beuy Joob, Viroj Wiwanitkit
Nature Medicine
Hannah Stower
© 2021 Meta ULC. All rights reserved