An influenza virus-triggered SUMO switch orchestrates co-opted endogenous retroviruses to stimulate host antiviral immunity

Proceedings of the National Academy of Sciences of the United States of America
Nora SchmidtBenjamin G Hale

Abstract

Dynamic small ubiquitin-like modifier (SUMO) linkages to diverse cellular protein groups are critical to orchestrate resolution of stresses such as genome damage, hypoxia, or proteotoxicity. Defense against pathogen insult (often reliant upon host recognition of "non-self" nucleic acids) is also modulated by SUMO, but the underlying mechanisms are incompletely understood. Here, we used quantitative SILAC-based proteomics to survey pan-viral host SUMOylation responses, creating a resource of almost 600 common and unique SUMO remodeling events that are mounted during influenza A and B virus infections, as well as during viral innate immune stimulation. Subsequent mechanistic profiling focused on a common infection-induced loss of the SUMO-modified form of TRIM28/KAP1, a host transcriptional repressor. By integrating knockout and reconstitution models with system-wide transcriptomics, we provide evidence that influenza virus-triggered loss of SUMO-modified TRIM28 leads to derepression of endogenous retroviral (ERV) elements, unmasking this cellular source of "self" double-stranded (ds)RNA. Consequently, loss of SUMO-modified TRIM28 potentiates canonical cytosolic dsRNA-activated IFN-mediated defenses that rely on RIG-I, MAVS, TBK1...Continue Reading

References

Jul 11, 2006·Retrovirology·Christoffer NellåkerHåkan Karlsson
Jul 27, 2007·Nature·Krishnamurthy MalathiRobert H Silverman
Jan 10, 2009·Nature Protocols·Da Wei HuangRichard A Lempicki
Apr 21, 2009·Nature Genetics·Geoffrey J FaulknerPiero Carninci
Jan 16, 2010·Nature·Helen M RoweDidier Trono
May 31, 2011·The Journal of Biological Chemistry·James M AraminiGaetano T Montelione
Jun 7, 2011·Nature Structural & Molecular Biology·Aaron A GoodarziPenelope A Jeggo
Sep 24, 2011·The Journal of Immunology : Official Journal of the American Association of Immunologists·Qiming LiangFanxiu Zhu
Oct 30, 2012·Bioinformatics·Alexander DobinThomas R Gingeras
Nov 30, 2012·Nucleic Acids Research·Tanya BarrettAlexandra Soboleva
Apr 30, 2013·Nature Reviews. Microbiology·Roger D EverettBenjamin G Hale
Aug 21, 2013·PLoS Computational Biology·Michael LawrenceVincent J Carey
Oct 26, 2013·Nature Protocols·F Ann RanFeng Zhang
Feb 22, 2014·Annual Review of Immunology·William M SchneiderCharles M Rice
Apr 4, 2014·Bioinformatics·Anthony M BolgerBjoern Usadel
Jun 1, 2014·Genome Research·Priscilla TurelliDidier Trono
Jul 12, 2014·BMC Genomics·Steven W CriscioneNicola Neretti
Jul 31, 2014·Nature Methods·Neville E SanjanaFeng Zhang
Jun 24, 2015·Cell Division·Jorrit M Enserink
Sep 18, 2015·Journal of Virology·Sanna M MäkeläIlkka Julkunen
Nov 10, 2015·Cell Reports·Patricia DominguesBenjamin G Hale
Dec 15, 2015·Cell Host & Microbe·Shashank TripathiSumit K Chanda
Mar 31, 2016·Nature Reviews. Immunology·George Kassiotis, Jonathan P Stoye
Aug 31, 2016·Proceedings of the National Academy of Sciences of the United States of America·Minmin LiuPeter A Jones
Jan 24, 2017·Nature Structural & Molecular Biology·Ivo A HendriksMichael L Nielsen
Mar 3, 2017·The Journal of Experimental Medicine·Ming-Ming HuHong-Bing Shu
Apr 16, 2017·The Journal of Biological Chemistry·Charlotte Lässig, Karl-Peter Hopfner
Sep 10, 2017·The Journal of Cell Biology·Trinna L CuellarBenjamin Haley
Sep 14, 2017·Cell Reports·Patricia Domingues, Benjamin G Hale
Nov 29, 2017·Nature Immunology·Jessica J ChiangMichaela U Gack

❮ Previous
Next ❯

Citations

Sep 13, 2019·Journal of Virology·Yang Zhao, John Karijolich
Jun 5, 2020·International Journal of Molecular Sciences·Pier-Angelo TovoMassimiliano Bergallo
Aug 14, 2020·PLoS Pathogens·Elizabeth J FayRyan A Langlois
Sep 10, 2020·Viruses·Tongtong Zhu, Ana Fernandez-Sesma
Sep 30, 2020·Proceedings of the National Academy of Sciences of the United States of America·Jiajun HanHui Peng
Mar 24, 2020·Nature Reviews. Immunology·Jan Rehwinkel, Michaela U Gack
Jan 23, 2020·Life Science Alliance·Marissa G MacchiettoSteven S Shen
Aug 10, 2020·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jorn E StokAnnemarthe G van der Veen
Aug 25, 2020·Current Clinical Microbiology Reports·Maria I GiraldoRicardo Rajsbaum
Aug 12, 2020·Fly·Sushmitha HegdeGirish S Ratnaparkhi
Jan 6, 2021·International Journal of Molecular Sciences·Ramesh KumarSujatha Sunil
Mar 3, 2021·Porcine Health Management·Hamish A Salvesen, C Bruce A Whitelaw
Mar 27, 2021·Pathogens and Global Health·Hong-Yeoul Ryu
Mar 28, 2021·European Journal of Pediatrics·Pier-Angelo TovoMassimiliano Bergallo
Apr 23, 2021·Journal of Virology·Smitha Srinivasachar Badarinarayan, Daniel Sauter
Jun 3, 2021·Pharmaceuticals·Karen Giménez-Orenga, Elisa Oltra
Jun 20, 2021·Cellular Signalling·Yinfang WangPeng Zhang
Mar 27, 2021·Annual Review of Biochemistry·Tim KrischunsStephen Cusack
Jul 25, 2021·International Journal of Molecular Sciences·Pier-Angelo TovoMassimiliano Bergallo
Jul 31, 2021·Frontiers in Cellular and Infection Microbiology·Zhu-Xing JiXiu-Fan Liu
Dec 23, 2020·Journal of Proteome Research·Jinming ZhangNing Liu
Aug 29, 2021·Viruses·Maarit Suomalainen, Urs F Greber
Sep 10, 2021·The Journal of Immunology : Official Journal of the American Association of Immunologists·Qi LiChunyuan Zhao

❮ Previous
Next ❯

Datasets Mentioned

BETA
KU55933
PXD014136

Methods Mentioned

BETA
tandem-affinity purification
RNA-seq
transfection
FACS
Immunoprecipitation
Assay
PCR

Software Mentioned

DAVID
R package edgeR
Bioconductor
STAR
Trimmomatic
GraphPad Prism
R package GenomicRanges

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

European Journal of Cell Biology
Hendrik Poeck, Jürgen Ruland
Proceedings of the National Academy of Sciences of the United States of America
Guofeng ChengFrancis V Chisari
BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology
Sujin KimYoosik Kim
© 2021 Meta ULC. All rights reserved