An informatic framework for decoding protein complexes by top-down mass spectrometry

Nature Methods
Owen S SkinnerNeil L Kelleher

Abstract

Efforts to map the human protein interactome have resulted in information about thousands of multi-protein assemblies housed in public repositories, but the molecular characterization and stoichiometry of their protein subunits remains largely unknown. Here, we report a computational search strategy that supports hierarchical top-down analysis for precise identification and scoring of multi-proteoform complexes by native mass spectrometry.

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Citations

Apr 5, 2017·Journal of the American Society for Mass Spectrometry·Nicole A HaverlandNeil L Kelleher
Jan 7, 2017·Molecular Biology of the Cell·Luis F Montaño-GutierrezJuri Rappsilber
May 15, 2016·Molecular & Cellular Proteomics : MCP·Rafael D MelaniNeil L Kelleher
Feb 12, 2017·Journal of Proteomics·Luca FornelliNeil L Kelleher
Feb 2, 2018·Journal of the American Society for Mass Spectrometry·Mowei ZhouLjiljana Paša-Tolić
Nov 14, 2017·Nature Chemical Biology·Owen S SkinnerPhilip D Compton
Aug 31, 2016·Nature Methods·Vivien Marx
Oct 18, 2018·Chemistry : a European Journal·Huixin WangWilliam A Donald
Oct 31, 2017·Mass Spectrometry Reviews·Dirk NoltingAlexander Makarov
May 3, 2019·Proteomics·Leah V SchafferLloyd M Smith
Apr 19, 2019·F1000Research·John R Yates
Jan 17, 2019·Biochemical Society Transactions·Timothy M Allison, Cherine Bechara
Jul 1, 2020·PLoS Computational Biology·Mikkel Herholdt JensenCheemeng Tan
Feb 23, 2020·Biochemistry. Biokhimii︠a︡·E V PoverennayaE A Ponomarenko
Mar 24, 2018·Science·Grace E KenneyAmy C Rosenzweig
Jun 29, 2018·Nature Communications·Michiel van de WaterbeemdAlbert J R Heck
Oct 28, 2017·The Journal of Venomous Animals and Toxins Including Tropical Diseases·Rafael D MelaniGilberto B Domont
Oct 21, 2020·Biochemical Society Transactions·Lindsay K PinoBirgit Schilling
Dec 17, 2020·Analytical Chemistry·John P McGeeNeil L Kelleher
Apr 10, 2019·Journal of the American Society for Mass Spectrometry·Luis F SchachnerNeil L Kelleher
Jan 5, 2021·Journal of the American Society for Mass Spectrometry·Zhe ZhangYang Yang
Sep 25, 2017·Analytical Chemistry·Owen S SkinnerNeil L Kelleher
Mar 28, 2017·Analytical Chemistry·Gili Ben-NissanMichal Sharon
Aug 19, 2021·Biochemistry·Xin ZengChu Wang
May 22, 2020·Journal of the American Society for Mass Spectrometry·Ashley N IvesNeil L Kelleher
Jan 24, 2020·Journal of the American Society for Mass Spectrometry·Jared O KafaderPhilip D Compton
Nov 23, 2017·Analytical Chemistry·Hui-Yin ChangTing-Yi Sung
Jul 12, 2017·Analytical Chemistry·Cheng-Chih HsuPieter C Dorrestein

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Methods Mentioned

BETA
acetylation

Software Mentioned

CORUM
NET MVC
ProSight
ProSight Lite
NET Framework
MPC
ASP
Xtract
mMass
TrEMBL

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