PMID: 19925448Nov 21, 2009Paper

An infrared reflection-absorption spectroscopic (IRRAS) study of the interaction of lipid A and lipopolysaccharide Re with endotoxin-binding proteins

Medicinal Chemistry
Andreas KerthKlaus Brandenburg

Abstract

Lipopolysaccharides (LPS, endotoxins) are main constituents of the outer membranes of Gram-negative bacteria, with the 'endotoxic principle' lipid A anchoring LPS into the membrane. When LPS is removed from the bacteria by the action of the immune system or simply by cell dividing, it may interact strongly with immunocompetent cells such as mononuclear cells. This interaction may lead, depending on the LPS concentration, to beneficial (at low) or pathophysiological (at high concentrations) reactions, the latter frequently causing the septic shock syndrome. There is a variety of endogenous LPS-binding proteins. To this class belong lactoferrin (LF) and hemoglobin (Hb), which have been shown to suppress and enhance the LPS-induced cytokine secretion in mononuclear cells, respectively. To elucidate the interaction mechanisms of endotoxins with these proteins, we have investigated in an infrared reflection-absorption spectroscopy (IRRAS) study the interaction of LPS or lipid A monolayers at the air/water interface with LF and Hb proteins, injected into the aqueous subphase. The data are clearly indicative of completely different interaction mechanisms of the endotoxins with the proteins, with the LF acting only at the LPS backbone,...Continue Reading

Citations

Jan 28, 2014·Journal of Pharmaceutical Sciences·Quinton M DowlingRyan M Kramer
Nov 22, 2011·International Immunopharmacology·Maria Elisa Drago-SerranoRafael Campos-Rodríguez
Jul 3, 2013·Biochimica Et Biophysica Acta·Alfred Blume, Andreas Kerth
Sep 12, 2020·European Biophysics Journal : EBJ·Martin RabePatrick Garidel
Nov 25, 2011·Chembiochem : a European Journal of Chemical Biology·Andreas KerthAlfred Blume
Jan 24, 2012·Langmuir : the ACS Journal of Surfaces and Colloids·Tina BrehmerAlfred Blume
Mar 30, 2013·Biomacromolecules·Shalini SinghMartin Malmsten

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