PMID: 8610122Apr 2, 1996Paper

An inhalational anesthetic binding domain in the nicotinic acetylcholine receptor

Proceedings of the National Academy of Sciences of the United States of America
Roderic G Eckenhoff

Abstract

To determine inhalational anesthetic binding domains on a ligand-gated ion channel, I used halothane direct photoaffinity labeling of the nicotinic acetylcholine receptor (nAChR) in native Torpedo membranes. [14C]Halothane photoaffinity labeling of both the native Torpedo membranes and the isolated nAChR was saturable, with Kd values within the clinically relevant range. All phospholipids were labeled, with greater than 95% of the label in the acyl chain region. Electrophoresis of labeled nAChR demonstrated no significant subunit selectivity for halothane incorporation. Within the alpha-subunit, greater than 90% of label was found in the endoprotease Glu-C digestion fragments which contain the four transmembrane regions, and the pattern was different from that reported for photoactivatable phospholipid binding to the nAChR. Unlabeled halothane reduced labeling more than did isoflurane, suggesting differences in the binding domains for inhalational anesthetics in the nAChR. These data suggest multiple similar binding domains for halothane in the transmembrane region of the nAChR.

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Citations

Sep 16, 1999·Cellular and Molecular Life Sciences : CMLS·M D Krasowski, N L Harrison
Mar 28, 2008·European Biophysics Journal : EBJ·M Weis, M Kopáni
May 29, 2000·Neuroscience Letters·M F Eckenhoff, R G Eckenhoff
Feb 19, 2002·Brain Research Bulletin·Edömer TassonyiDaniel Bertrand
Feb 27, 1999·Toxicology Letters·M BarannJ P Dilger
Feb 27, 1999·Toxicology Letters·A B WoskaD Scharf
Jul 28, 2010·Proceedings of the National Academy of Sciences of the United States of America·Grace BranniganMichael L Klein
Oct 4, 2006·Current Opinion in Anaesthesiology·J G Bovill
Aug 2, 2011·Biochemistry Research International·Lemont B Kier
Aug 26, 1998·Anesthesia and Analgesia·R G Eckenhoff
Jul 18, 2008·Anesthesia and Analgesia·Max B Kelz, Roderic G Eckenhoff
Jun 14, 2012·PLoS Computational Biology·David N LeBardGrace Brannigan
Feb 25, 2014·Current Anesthesiology Reports·Brian P WeiserRoderic G Eckenhoff
Jul 15, 2005·Best Practice & Research. Clinical Anaesthesiology·George A MashourJason A Campagna
Nov 13, 2007·Biophysical Journal·Vasyl BondarenkoPei Tang
Mar 23, 2004·Journal of Chemical Information and Computer Sciences·Adolf Miklavc, Darko Kocjan
Sep 25, 2012·Biochimica Et Biophysica Acta·Vasyl BondarenkoPei Tang
Sep 23, 2010·Biophysical Journal·Qiang ChenPei Tang
Oct 19, 2011·Biophysical Journal·Dan WillenbringPei Tang
Aug 1, 2006·Biophysical Journal·John H StreiffKeith A Jones
Feb 26, 1998·Anesthesiology·C W HönemannM E Durieux
May 2, 2008·Clinical Pharmacology and Therapeutics·Rg EckenhoffMb Kelz
Apr 2, 2004·European Journal of Anaesthesiology·E MasakiM Kawamura
Dec 26, 2001·The Journal of Pharmacology and Experimental Therapeutics·Maryellen Fazen EckenhoffRoderic G Eckenhoff
Feb 28, 2004·The Journal of Biological Chemistry·Jin XiRoderic G Eckenhoff
May 17, 2012·Chemistry & Biodiversity·Lemont B Kier, Patricia W Slattum
Jun 28, 1996·The Journal of Biological Chemistry·R G Eckenhoff
Oct 1, 2009·Accounts of Chemical Research·Satyavani VemparalaMichael L Klein

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