An inhibitor-based method to measure initial decomposition of naturally occurring polysaccharides in sediments.

Applied and Environmental Microbiology
H BoschkerT E Cappenberg

Abstract

A method that can be used to measure the initial decomposition rates of polysaccharides in sediment samples was developed. It uses toluene to specifically inhibit microbial uptake of carbohydrates without affecting extracellular hydrolysis of polysaccharides. Accumulating carbohydrates were determined by high-performance liquid chromatography. Field-sampled litter from the common reed (Phragmites australis), which contains cellulose and arabinoxylan as its main polysaccharides, was used as a model system. Toluene concentrations of between 1 and 10% resulted in the accumulation of similar amounts of monomeric carbohydrates, which was linear over time for most neutral sugars. Toluene (3%) did not have an effect on extracellular enzyme activities, and microbial sugar uptake was completely inhibited, as demonstrated with (sup14)C-labelled xylose and glucose. Experiments with enhancement cultures and fixed reed litter suggested that enzymatic hydrolysis of polysaccharides in reed litter was the main source of glucose, xylose, arabinose, and galactose accumulation. In contrast, the accumulation of high amounts of the alditols mannitol and glucitol was probably caused by lysis of the microbial population in toluene-treated reed litter...Continue Reading

References

Sep 1, 1988·Microbiological Reviews·K K WongJ N Saddler
Mar 1, 1982·Analytical Biochemistry·H Felix
Oct 1, 1984·Applied and Environmental Microbiology·G Bratbak, I Dundas
Sep 24, 1982·Science·P H YanceyG N Somero
Jan 1, 1982·Applied and Environmental Microbiology·D F Toerien, B Cavari
Dec 1, 1982·Applied and Environmental Microbiology·G M King, M J Klug
Feb 1, 1986·Applied and Environmental Microbiology·G M King
Aug 1, 1989·Applied and Environmental Microbiology·H W Cunningham, Robert G Wetzel
Jan 1, 1993·Applied and Environmental Microbiology·T E Sawyer, G M King

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