PMID: 2118625Aug 25, 1990Paper

An inhibitor of DNA polymerases alpha and delta in calf thymus DNA

Nucleic Acids Research
M Goulian, C J Heard

Abstract

Most, although not all, samples of commercial calf thymus DNA were strongly inhibitory to DNA polymerase alpha; the inhibition made the DNA useless as a template for this enzyme. In a pre-assembled DNA polymerase assay mixture (minus enzyme but including activated DNA) the inhibition tended to diminish with time but at a rate that was not predictable, and some inhibition usually persisted. It was concluded that the inhibition was the result of contamination of the DNA by a heparin-like material on the basis of the following: 1) the inhibition could be reversed by treatment of the DNA with heparinase; 2) both the endogenous inhibitory effect of calf thymus DNA as well as the inhibitory effect of heparin on DNA polymerase alpha are reversed by protamine (which is known to prevent the antithrombin activity of heparin); 3) both the endogenous inhibition and inhibition by heparin are also reversed by ampholyte (which also prevents the antithrombin activity of heparin); and 4) both the endogenous and the heparin-induced inhibitory effects display the same spectrum of activity against mammalian DNA polymerases, i.e. both DNA polymerases alpha and delta are extremely sensitive whereas, DNA polymerases beta and gamma are resistant. The ...Continue Reading

References

Jan 1, 1989·Annual Review of Biochemistry·M D Challberg, T J Kelly
Jan 1, 1989·Annual Review of Cell Biology·B Stillman
Apr 2, 1987·Nature·R BravoH Macdonald-Bravo
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