An innovative silicon-chip for sensitive real time PCR improvement in pathogen detection

The Analyst
Simone BattagliaSabrina Conoci

Abstract

An innovative miniaturized silicon-chip was developed for highly sensitive detection of pathogen genomes of both viruses and bacteria through real time PCR (qRT-PCR). The device was properly designed to enhance the optical signal and perform accurate thermal control. Results show an improvement of PCR amplification by one order of magnitude in sensitivity compared to the standard RT-PCR method. In particular for hepatitis B virus a decrease of the mean value of Ct of about 2.9 ± 0.9 compared to the standard system was observed. Similarly, for the bacteria Pseudomonas aeruginosa, Staphylococcus aureus and Acinetobacter baumannii, a decrease of the mean values of Ct of 1.8 ± 0.5, 3.1 ± 0.5 and 3.9 ± 0.9, respectively, was observed.

References

Jan 13, 2010·Clinical Microbiology Reviews·Nicasio ManciniMassimo Clementi
Sep 24, 2013·Journal of Microbiological Methods·B J Parcell, G V Orange
Jan 8, 2014·Annals of Clinical Microbiology and Antimicrobials·Hye-Young WangHyeyoung Lee
Dec 18, 2016·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·C WattalS Khanna
Feb 12, 2017·Applied Biochemistry and Biotechnology·Dayeseul Lim, Jae Chern Yoo
Apr 12, 2017·Sensors·Maria GuarnacciaSebastiano Cavallaro
Jul 29, 2017·ACS Sensors·Salvatore Petralia, Sabrina Conoci

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Methods Mentioned

BETA
nucleic
PCR
biosensor
biosensing
chip
thermal exchange

Software Mentioned

BioEdit
BLAST
ClustalW
IDYLLA
Spartan
Bio
primer express
Revogene

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