An insert in the covS gene distinguishes a pharyngeal and a blood isolate of Streptococcus pyogenes found in the same individual.

Microbiology
Alan F GarciaKaren Yamaga

Abstract

Expression of the extensive arsenal of virulence factors by Streptococcus pyogenes is controlled by many regulators, of which CovRS is one of the best characterized and can influence ∼15 % of the genome. Animal models have established that mutants of covRS arise spontaneously in vivo resulting in highly invasive organisms. We analysed a pharyngeal and a blood isolate of S. pyogenes recovered from the same individual 13 days apart. The two isolates varied in many phenotypic properties including SpeB production, which were reflected in transcriptomic analyses. PFGE, multilocus sequence typing and partial sequencing of some key genes failed to show any differences except for an 11 bp insert in the covS gene in the blood isolate which caused a premature termination of transcription. Complementation of a fully functional covS gene into the blood isolate resulted in high expression of CovS and expression of speB. These results, showing a pharyngeal and a blood isolate from a single individual differing by a simple insertion, provide evidence for the model that regulatory gene mutations allow S. pyogenes to invade different niches in the body.

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Citations

Mar 19, 2011·Current Opinion in Infectious Diseases·Nicola N LynskeyShiranee Sriskandan
Dec 3, 2014·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Debra E BessenHervé Tettelin
Sep 17, 2011·Nature Reviews. Microbiology·Jason N ColeMark J Walker
Mar 28, 2013·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·James A TsatsaronisMartina L Sanderson-Smith

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Datasets Mentioned

BETA
GSE21316

Methods Mentioned

BETA
PCR
electrophoresis
chips

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