An Insight into the Constitutive Proteome Throughout Leishmania donovani Promastigote Growth and Differentiation

International Microbiology : the Official Journal of the Spanish Society for Microbiology
Pedro J AlcoleaVicente Larraga

Abstract

Anthroponotic visceral leishmaniasis is a life-threatening disease caused by Leishmania donovani (Kinetoplastida: Trypanosomatidae) in East Africa and the Indian subcontinent. Unlike promastigote growth and differentiation in the sand fly gut or in axenic culture, L. donovani promastigote-into-amastigote development has been studied by high-throughput gene expression profiling. In this study, we have identified abundant constitutive proteins in axenically cultured promastigotes by two-dimension electrophoresis and matrix-assisted laser desorption-ionization tandem time-of-flight (MALDI-TOF/TOF) mass spectrometry. Most proteins involved in the trypanothione-based redox antioxidant system are expressed constitutively throughout axenic L. donovani promastigote growth and differentiation (tryparedoxin, trypanothione peroxidase, generic peroxidoxin, iron-superoxide dismutase, and elongation factor 1β). These findings are in agreement with previous data on other Old World species (i.e., L. major and L. infantum), whereas New World species (i.e., L. amazonensis and L. pifanoi) and Crithidia fasciculata show different expression patterns.

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Datasets Mentioned

BETA
PXD009011

Methods Mentioned

BETA
electrophoresis
Protein Assay
protein folding

Software Mentioned

Global Protein Server Explorer
MASCOT
Edit Spot Tools
ABI 4000 Series Explorer
PDQuest

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