PMID: 11329613May 1, 2001Paper

An insight into the mechanism of cytotoxicity of ricin to hepatoma cell: roles of Bcl-2 family proteins, caspases, Ca(2+)-dependent proteases and protein kinase C

Journal of Cellular Biochemistry
R HuX Liu

Abstract

The ability of ricin, a type II ribosome-inactivating protein, to induce hepatoma cell (BEL7404) to apoptosis in vitro was examined by fluorescence microscopy, flow cytometry, and DNA fragmentation assay. As a Bcl-2 lacking model, BEL7404 bore unique advantage to study the effect of over-expressing Bcl-2 on the apoptosis induced by the inhibitor of protein synthesis. By establishing a Bcl-2 over-expressing cell line (BEL7404/ Bcl-2), we found that Bcl-2 could promote the survival of the hepatoma cell against ricin insult. The ricin-induced apoptosis of BEL7404 was accompanied by increased expression of Bak and decreased levels of Bcl-xl and Bax. Caspases and PARP cleavage activity were found to be implicated in the death process. Through the inhibitor tests, our results excluded the participation of calcium-dependent proteases or protein kinase C in the apoptotic process induced by ricin, though an elevation of intracellular calcium did occur as an immediate response to ricin treatment. Cycloheximide, another protein synthesis inhibitor, did synergistically enhance rather than inhibit the cytotoxicity of ricin to hepatoma cell BEL7404. Actually, cycloheximide alone was able to induce hepatoma cell BEL7404 to death that could al...Continue Reading

References

Jun 1, 1992·Experimental Cell Research·K Sandvig, B van Deurs
Jan 1, 1994·Methods in Cell Biology·J P Kao
Dec 21, 1993·Biochimica Et Biophysica Acta·L BarbieriF Stirpe
Jan 1, 1996·Leukemia & Lymphoma·M Ohh, F Takei
Apr 28, 1997·Toxicology Letters·J M WilliamsC M Taylor
Nov 5, 1997·Biochemical and Biophysical Research Communications·G ChenS F Moss
Jan 1, 1997·British Medical Bulletin·C O Bellamy
Jul 21, 1998·Annals of the New York Academy of Sciences·N L BanikE L Hogan
Aug 28, 1998·Science·N A Thornberry, Y Lazebnik
Sep 12, 1998·Science·J M Adams, S Cory
Dec 4, 1998·Biochemistry·A Keppler-HafkemeyerI Pastan
Oct 26, 1999·Infection and Immunity·C M ThorpeD W Acheson
Mar 22, 2000·Proceedings of the National Academy of Sciences of the United States of America·B Li, Q P Dou
May 1, 1998·Annals of the New York Academy of Sciences·N L BanikE L Hogan

❮ Previous
Next ❯

Citations

Mar 5, 2004·Toxicology and Applied Pharmacology·Hideki OhbaNobuyuki Yamasaki
Jun 5, 2003·Toxicon : Official Journal of the International Society on Toxinology·Luis DaSilvaMark Dertzbaugh
Feb 21, 2002·The International Journal of Biochemistry & Cell Biology·Rong-Gui HuWang-Yi Liu
Mar 1, 2005·FEBS Letters·Sriram NarayananAnjali A Karande
Jul 15, 2015·International Journal of Molecular Medicine·Chengzhi XieXundi Xu
Jul 10, 2008·The International Journal of Biochemistry & Cell Biology·Deepa SikriwalJanendra K Batra
May 6, 2014·Acta Pharmacologica Sinica·Subhadip MukhopadhyaySujit Kumar Bhutia
Oct 29, 2003·Toxicological Reviews·Michael J LordLynne M Roberts
Sep 27, 2005·Critical Reviews in Food Science and Nutrition·Elvira González De Mejía, Valentin I Prisecaru
Apr 25, 2013·Biological Chemistry·Sangeeta MehtaSujata Ghosh
Mar 5, 2002·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Maurizio BrigottiSimonetta Sperti
Jun 21, 2019·Toxins·Natalia Sowa-RogozińskaMonika Słomińska-Wojewódzka
Aug 11, 2004·Toxicon : Official Journal of the International Society on Toxinology·Fiorenzo Stirpe

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptotic Caspases

Apoptotic caspases belong to the protease enzyme family and are known to play an essential role in inflammation and programmed cell death. Here is the latest research.

BCL-2 Family Proteins

BLC-2 family proteins are a group that share the same homologous BH domain. They play many different roles including pro-survival signals, mitochondria-mediated apoptosis and removal or damaged cells. They are often regulated by phosphorylation, affecting their catalytic activity. Here is the latest research on BCL-2 family proteins.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis