An insight into the sialotranscriptome of the brown dog tick, Rhipicephalus sanguineus.

BMC Genomics
Elen AnatrielloBeatriz R Ferreira

Abstract

Rhipicephalus sanguineus, known as the brown dog tick, is a common ectoparasite of domestic dogs and can be found worldwide. R.sanguineus is recognized as the primary vector of the etiological agent of canine monocytic ehrlichiosis and canine babesiosis. Here we present the first description of a R. sanguineus salivary gland transcriptome by the production and analysis of 2,034 expressed sequence tags (EST) from two cDNA libraries, one consctructed using mRNA from dissected salivary glands from female ticks fed for 3-5 days (early to mid library, RsSGL1) and the another from ticks fed for 5 days (mid library, RsSGL2), identifying 1,024 clusters of related sequences. Based on sequence similarities to nine different databases, we identified transcripts of genes that were further categorized according to function. The category of putative housekeeping genes contained approximately 56% of the sequences and had on average 2.49 ESTs per cluster, the secreted protein category contained 26.6% of the ESTs and had 2.47 EST's/clusters, while 15.3% of the ESTs, mostly singletons, were not classifiable, and were annotated as "unknown function". The secreted category included genes that coded for lipocalins, proteases inhibitors, disintegrin...Continue Reading

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Citations

Sep 14, 2013·Ticks and Tick-borne Diseases·C J OliveiraJ M C Ribeiro
Mar 3, 2011·BMC Genomics·José Mc RibeiroIvo Mb Francischetti
Jul 13, 2012·BMC Genomics·María Estrella SantamaríaManuel Martinez
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Oct 17, 2017·Frontiers in Cellular and Infection Microbiology·Sandra AntunesAna Domingos

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Datasets Mentioned

BETA
GT030184
GT032391

Methods Mentioned

BETA
PCR
size fractionation
glycosylation

Software Mentioned

ENSEMBL
Mega package
BLASTx
ClustalX
Clustal
BLASTn
rpsBLAST
NetOGlyc
Count Libraries
Excel

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