An integrated high resolution mass spectrometric data acquisition method for rapid screening of saponins in Panax notoginseng (Sanqi)

Journal of Pharmaceutical and Biomedical Analysis
Chang-Jiang-Sheng LaiE-Hu Liu

Abstract

The aim of this study was to develop a convenient method without pretreatments for nontarget discovery of interested compounds. The segment and exposure strategy, coupled with two mass spectrometer data acquisition methods was firstly proposed for screening the saponins in extract of Panax notoginseng (Sanqi) via high-performance liquid chromatography tandem quadrupole time-of-flight mass spectrometry (HPLC-QTOF/MS). By gradually removing certain major or moderate interference compounds, the developed segment and exposure strategy could significantly improve the detection efficiency for trace compounds. Moreover, the newly developed five-point screening approach based on a modified mass defect filter strategy and the visual isotopic ion technique was verified to be efficient and reliable in picking out the interested precursor ions. In total, 234 ginsenosides including 67 potential new ones were characterized or tentatively identified from the extract of Sanqi. Particularly, some unusual compounds containing the branched glycosyl group or new substituted acyl groups were firstly reported. The proposed integrated strategy held a strong promise for analyses of the complex mixtures.

References

Feb 10, 2004·Journal of the American Society for Mass Spectrometry·Shuying LiuWenjun Mo
Jan 24, 2007·Rapid Communications in Mass Spectrometry : RCM·Eric C Y ChanHwee-Ling Koh
Feb 26, 2009·Rapid Communications in Mass Spectrometry : RCM·Russell J Mortishire-SmithAlastair Hill
Nov 26, 2010·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Li HeDong Zhou
Jan 25, 2011·Natural Product Reports·Lian-Wen QiChun-Su Yuan
May 19, 2011·Mass Spectrometry Reviews·Dirk ValkenborgTomasz Burzykowski
Feb 22, 2012·Journal of the American Society for Mass Spectrometry·Jürgen ClaesenDirk Valkenborg
Jul 17, 2012·Journal of Pharmaceutical and Biomedical Analysis·Hui ZhangPing Li
May 15, 2013·Analytical Chemistry·Lars RidderJacques Vervoort
Jun 26, 2013·Journal of Chromatography. a·Tairo OguraEiichiro Fukusaki
Feb 4, 2014·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Minyan LiuPengfei Tu
Feb 13, 2014·Journal of the American Society for Mass Spectrometry·Piotr Dittwald, Dirk Valkenborg

❮ Previous
Next ❯

Citations

Apr 24, 2016·Journal of Pharmaceutical and Biomedical Analysis·Jimmy K LeluPing Li
May 26, 2017·Evidence-based Complementary and Alternative Medicine : ECAM·Bashir Mohammed AbubakarAlina Wagiran
Sep 12, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Lili HuangMingqing Huang
Jan 14, 2021·Journal of Ginseng Research·Yuangui YangZhengtao Wang

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.