An integrated hybrid microfluidic device for oviposition-based chemical screening of adult Drosophila melanogaster

Lab on a Chip
Jacob C K LeungPouya Rezai

Abstract

Chemical screening using Drosophila melanogaster (the fruit fly) is vital in drug discovery, agricultural, and toxicological applications. Oviposition (egg laying) on chemically-doped agar plates is an important read-out metric used to quantitatively assess the biological fitness and behavioral responses of Drosophila. Current oviposition-based chemical screening studies are inaccurate, labor-intensive, time-consuming, and inflexible due to the manual chemical doping of agar. In this paper, we have developed a novel hybrid agar-polydimethylsiloxane (PDMS) microfluidic device for single- and multi-concentration chemical dosing and on-chip oviposition screening of free-flying adult stage Drosophila. To achieve this, we have devised a novel technique to integrate agar with PDMS channels using ice as a sacrificial layer. Subsequently, we have conducted single-chemical toxicity and multiple choice chemical preference assays on adult Drosophila melanogaster using zinc and acetic acid at various concentrations. Our device has enabled us to 1) demonstrate that Drosophila is capable of sensing the concentration of different chemicals on a PDMS-agar microfluidic device, which plays significant roles in determining oviposition site select...Continue Reading

References

Feb 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·A SehgalM W Young
Apr 1, 2000·Science·R G WalkerC S Zuker
Oct 14, 2000·Cell Research·C Y Lee, E H Baehrecke
Oct 23, 2002·Proceedings of the National Academy of Sciences of the United States of America·Frederic Mery, Tadeusz J Kawecki
Jun 24, 2004·Cell·Zuoren WangKristin Scott
Jul 9, 2004·Journal of Agricultural and Food Chemistry·Mitsuo MiyazawaYukio Ishikawa
Nov 6, 2004·Gene·Ethan Bier, Rolf Bodmer
Jan 5, 2005·Nature Reviews. Genetics·Ethan Bier
Aug 27, 2005·Proceedings of the National Academy of Sciences of the United States of America·Michael R BogwitzPhillip J Daborn
Oct 22, 2005·Mental Retardation and Developmental Disabilities Research Reviews·Linda L Restifo
Apr 17, 2007·Pflügers Archiv : European journal of physiology·Maurice J Kernan
Feb 6, 2008·The Review of Scientific Instruments·E CornellJ Jin
Jun 23, 2009·Proceedings of the National Academy of Sciences of the United States of America·Ryan M JosephUlrike Heberlein
Aug 8, 2009·Current Opinion in Neurobiology·Craig Montell
Jan 22, 2010·Lab on a Chip·Sarvesh Upadhyaya, P Ravi Selvaganapathy
Feb 2, 2011·PloS One·Paige M MillerThomas L Turner
Mar 19, 2011·Pharmacological Reviews·Udai Bhan Pandey, Charles D Nichols
Jan 1, 2005·Integrative and Comparative Biology·Thomas G Wilson
Dec 20, 2011·Virology·Tamara T HughesJoseph Ahlander
Mar 16, 2013·Nature Protocols·Thomas J LevarioHang Lu
Dec 10, 2013·Current Biology : CB·Hany K M DweckMarcus C Stensmyr
Dec 18, 2013·Chronobiology International·Anuj MenonVijay Kumar Sharma

❮ Previous
Next ❯

Citations

Feb 22, 2018·Biomicrofluidics·Amir Reza PeimaniPouya Rezai
Jan 23, 2020·Integrative Biology : Quantitative Biosciences From Nano to Macro·Alireza ZabihihesariPouya Rezai

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.