PMID: 8610181Apr 2, 1996Paper

An integrated map of the genome of the tubercle bacillus, Mycobacterium tuberculosis H37Rv, and comparison with Mycobacterium leprae

Proceedings of the National Academy of Sciences of the United States of America
W J PhilippS T Cole

Abstract

An integrated map of the genome of the tubercle bacillus, Mycobacterium tuberculosis, was constructed by using a twin-pronged approach. Pulsed-field gel electrophoretic analysis enabled cleavage sites for Asn I and Dra I to be positioned on the 4.4-Mb circular chromosome, while, in parallel, clones from two cosmid libraries were ordered into contigs by means of fingerprinting and hybridization mapping. The resultant contig map was readily correlated with the physical map of the genome via the landmarked restriction sites. Over 165 genes and markers were localized on the integrated map, thus enabling comparisons with the leprosy bacillus, Mycobacterium leprae, to be undertaken. Mycobacterial genomes appear to have evolved as mosaic structures since extended segments with conserved gene order and organization are interspersed with different flanking regions. Repetitive sequences and insertion elements are highly abundant in M. tuberculosis, but the distribution of IS6110 is apparently nonrandom.

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Citations

Aug 12, 1998·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·K von FiguraT Dierks
Jan 7, 1998·Biochemical Pharmacology·C E Barry
May 5, 2001·Pharmacology & Therapeutics·A BzowskaD Shugar
Jul 9, 1999·Biochimica Et Biophysica Acta·L Philippot, O Højberg
Mar 20, 1999·Biochimica Et Biophysica Acta·M H SaierG B Young
May 22, 2003·FEMS Microbiology Letters·Irene HorneJohn G Oakeshott
Jun 24, 2000·Research in Microbiology·R BroschS T Cole
Oct 10, 1998·Research in Microbiology·C Boursaux-EudeI Saints Girons
Dec 1, 1996·Tubercle and Lung Disease : the Official Journal of the International Union Against Tuberculosis and Lung Disease·S T Cole
Sep 13, 2001·Trends in Microbiology·R BroschS T Cole
Jan 1, 1997·Molecular Medicine Today·D B Young
Jul 17, 1998·Current Opinion in Chemical Biology·H D Mootz, M A Marahiel
Mar 6, 1999·Current Opinion in Microbiology·S T Cole
Oct 25, 2000·International Journal of Medical Microbiology : IJMM·R BroschS T Cole
Aug 28, 1998·Molecular Microbiology·M GomezI Smith
Apr 23, 2002·Molecular Microbiology·Sayera BanuStewart T Cole
Feb 24, 2001·Environmental Microbiology·T H SmitsJ B van Beilen
Sep 22, 1998·The Journal of Experimental Medicine·B R OttoB Oudega
Aug 2, 2006·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Bichitra K BiswalMichael N G James
May 6, 2011·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Sangwoo KimKyung Jin Kim
Mar 1, 1997·European Journal of Biochemistry·P PeirsJ Content
Jan 5, 2000·Applied and Environmental Microbiology·A JesenskáJ Damborský
Jun 29, 2002·Applied and Environmental Microbiology·Irene HorneJohn G Oakeshott

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