An Integrated Multi-omic Single-Cell Atlas of Human B Cell Identity.

Immunity
David R GlassSean C Bendall

Abstract

B cells are capable of a wide range of effector functions including antibody secretion, antigen presentation, cytokine production, and generation of immunological memory. A consistent strategy for classifying human B cells by using surface molecules is essential to harness this functional diversity for clinical translation. We developed a highly multiplexed screen to quantify the co-expression of 351 surface molecules on millions of human B cells. We identified differentially expressed molecules and aligned their variance with isotype usage, VDJ sequence, metabolic profile, biosynthesis activity, and signaling response. Based on these analyses, we propose a classification scheme to segregate B cells from four lymphoid tissues into twelve unique subsets, including a CD45RB+CD27- early memory population, a class-switched CD39+ tonsil-resident population, and a CD19hiCD11c+ memory population that potently responds to immune activation. This classification framework and underlying datasets provide a resource for further investigations of human B cell identity and function.

References

Apr 20, 2001·Proceedings of the National Academy of Sciences of the United States of America·V G TusherG Chu
May 23, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Woong-Jai Won, John F Kearney
Jan 9, 2003·European Journal of Immunology·Peter J MacardleHeddy Zola
Jan 21, 2006·Nature Immunology·Taro Kawai, Shizuo Akira
May 8, 2007·Cell·Marcus E PeterDavid H Lynch
Jan 22, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Andrew C VendelDan L Eaton
Aug 12, 2009·Proceedings of the National Academy of Sciences of the United States of America·Mirzokhid RakhmanovKlaus Warnatz
Oct 16, 2009·Annual Review of Immunology·Tomohiro KurosakiYoshihiro Baba
Oct 27, 2009·Nature Immunology·Ismail DoganJean-Claude Weill
Jun 15, 2010·Proceedings of the National Academy of Sciences of the United States of America·Jonathan M IrishRonald Levy
Nov 17, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Mary M TomaykoMark J Shlomchik
Jan 10, 2012·Annual Review of Immunology·Gabriel D Victora, Michel C Nussenzweig
Feb 22, 2012·Nature Reviews. Immunology·Holden T MaeckerRobert Nussenblatt
Mar 8, 2012·Infectious Disorders Drug Targets·Takeshi Tsubata
Oct 23, 2012·Frontiers in Immunology·Denise A KaminskiIgnacio Sanz
Mar 21, 2013·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Rachel FinckSean C Bendall
Apr 4, 2013·Blood·Dora PintoDavid Jarrossay
Apr 23, 2013·Trends in Molecular Medicine·Luca AntonioliGyörgy Haskó
May 15, 2013·Nucleic Acids Research·Jian YeJames M Ostell
Oct 12, 2013·Nature Reviews. Immunology·Vanja LazarevicGraham M Lord
Mar 13, 2014·Bioinformatics·Jason A Vander HeidenSteven H Kleinstein
Jun 2, 2014·Nature Immunology·Griselda V Zuccarino-CataniaMark J Shlomchik
Nov 8, 2014·Nucleic Acids Research·Marie-Paule LefrancSofia Kossida
Feb 24, 2015·Nature Reviews. Immunology·Stephen L NuttLynn M Corcoran
Jun 13, 2015·Bioinformatics·Namita T GuptaSteven H Kleinstein
Mar 25, 2016·Clinical and Experimental Immunology·K ThorarinsdottirI-L Mårtensson
Apr 12, 2016·Current Opinion in Immunology·Scott D Boyd, James E Crowe
Aug 2, 2017·Cellular Immunology·Jodi L KarnellRachel Ettinger
Jul 19, 2018·Scientific Reports·Felix J HartmannSean C Bendall
Mar 14, 2019·Nature Communications·Samuel C KimmeySean C Bendall
Apr 9, 2019·The Journal of Allergy and Clinical Immunology·Rodrigo Jiménez-SaizManel Jordana
Apr 20, 2019·Cell·Jason G Cyster, Christopher D C Allen
Jul 18, 2019·Cell Reports·Felix J HartmannSean C Bendall

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Citations

Dec 9, 2020·Allergy·Irene SoletoYolanda R Carrasco
Dec 22, 2020·Frontiers in Immunology·Víctor A Sosa-HernándezJosé L Maravillas-Montero
May 14, 2021·Pain Reports·Michael J LacagninaPeter M Grace
May 13, 2021·Experimental Hematology·Ignacio Campillo-MarcosManel Esteller
Jul 6, 2021·JACC. Basic to Translational Science·Tanyaporn PattarabanjirdColeen McNamara
Jun 18, 2021·ImmunoTargets and Therapy·Joshua F E KoenigManel Jordana
Jul 30, 2021·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·M HelmR Freitag
Jun 26, 2021·Pediatric Allergy and Immunology : Official Publication of the European Society of Pediatric Allergy and Immunology·Sean R HolmCatherine A Thornton
Aug 28, 2021·Cardiovascular Research·Florentina PorschChristoph J Binder

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Methods Mentioned

BETA
fluorescence-activated cell sorting
glycosylation
biopsy
density gradient centrifugation
Assay

Software Mentioned

umap
Cytobank
FlowSOM
R
Generic Term Mapper
based
Adaptive Biotechnology
uwot
MATLAB
Bioconductor

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