An integrated, multiparametric flow cytometry chip using "microfluidic drifting" based three-dimensional hydrodynamic focusing.

Biomicrofluidics
Xiaole MaoTony Jun Huang

Abstract

In this work, we demonstrate an integrated, single-layer, miniature flow cytometry device that is capable of multi-parametric particle analysis. The device integrates both particle focusing and detection components on-chip, including a "microfluidic drifting" based three-dimensional (3D) hydrodynamic focusing component and a series of optical fibers integrated into the microfluidic architecture to facilitate on-chip detection. With this design, multiple optical signals (i.e., forward scatter, side scatter, and fluorescence) from individual particles can be simultaneously detected. Experimental results indicate that the performance of our flow cytometry chip is comparable to its bulky, expensive desktop counterpart. The integration of on-chip 3D particle focusing with on-chip multi-parametric optical detection in a single-layer, mass-producible microfluidic device presents a major step towards low-cost flow cytometry chips for point-of-care clinical diagnostics.

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Citations

Nov 13, 2012·Analytical Chemistry·Feng GuoTony Jun Huang
Dec 18, 2013·ACS Nano·Mengqian LuTony Jun Huang
Jan 11, 2014·Lab on a Chip·Yuchao ChenTony Jun Huang
Jan 11, 2014·Lab on a Chip·Yuchao ChenTony Jun Huang
Feb 4, 2014·Lab on a Chip·Menake E Piyasena, Steven W Graves
Dec 3, 2013·Analytical and Bioanalytical Chemistry·Thomas HaselgrüblerJan Hesse
Jun 27, 2014·Lab on a Chip·Daniel SpencerHywel Morgan
Sep 13, 2012·Lab on a Chip·Xiaole Mao, Tony Jun Huang
Aug 1, 2013·Lab on a Chip·Xiaoyun DingTony Jun Huang
Sep 4, 2015·Analytical Chemistry·Ahmad Ahsan NawazTony Jun Huang
Oct 19, 2013·Microscopy Research and Technique·Raju RegmiPartha P Mondal
May 23, 2015·FEMS Microbiology Reviews·Mark A LeverBo Barker Jørgensen
Feb 7, 2015·Biomedical Optics Express·Genni TestaRomeo Bernini
Mar 13, 2015·Lab on a Chip·Xiao WangIan Papautsky
Jul 22, 2014·Biosensors & Bioelectronics·Michael A DanieleFrances S Ligler
May 18, 2016·Analytical Chemistry·Sixing LiTony Jun Huang
Jun 1, 2016·Lab on a Chip·Daniel SpencerHywel Morgan
Sep 15, 2016·Sensors·Jingjing Zhao, Zheng You
Feb 23, 2017·Lab on a Chip·Adele De NinnoFederica Caselli
Aug 21, 2015·Lab on a Chip·Liqiang RenTony Jun Huang
Oct 9, 2014·The Analyst·Evgenia Yu Basova, Frantisek Foret
Sep 23, 2018·Electrophoresis·Yanli GongHai Jiang
Sep 25, 2014·Journal of Laboratory Automation·Zhangming MaoTony Jun Huang
Jun 5, 2014·Annals of Biomedical Engineering·Ahmad Ahsan NawazTony Jun Huang
Jun 18, 2017·Scientific Reports·Eric PedrolRamon A Alvarez-Puebla
Sep 29, 2017·Scientific Reports·Mohammad AsghariCaglar Elbuken
Jul 6, 2019·Frontiers in Bioengineering and Biotechnology·Abhishek VembadiMohammad A Qasaimeh
Dec 11, 2019·Scientific Reports·Usama AbbasiV Kumaran
May 21, 2020·Biomedical Engineering Letters·Aakash Rajawat, Siddhartha Tripathi
Jan 9, 2018·Chemical Society Reviews·Hui Yang, Martin A M Gijs
Nov 10, 2012·Lab on a Chip·Yanhui ZhaoTony Jun Huang
Oct 31, 2015·RSC Advances·Robert ZmijanPeter Glynne-Jones

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Methods Mentioned

BETA
flow cytometry
flow
chip

Software Mentioned

MATLAB

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