An integrated physical and genetic map of a 35 Mb region on chromosome Xp22.3-Xp21.3

Human Molecular Genetics
G B FerreroA Ballabio

Abstract

We have constructed a detailed physical map of the 35 Mb region spanning human chromosome Xp22.3-Xp21.3. The backbone of the map is represented by a single oriented contiguous stretch of 585 overlapping yeast artificial chromosome (YAC) clones covering the entire region. The map is formatted with 615 map objects that include 324 YACs, 185 sequence tagged sites, 28 genes, 85 chromosomal breakpoints and 37 highly polymorphic markers. Physical mapping was both guided and confirmed using 183 bins defined by chromosomal breakpoints and by overlapping regions of YAC clones. The localization of polymorphic markers in the physical map permits the integration of physical and genetic data across the region. These data establish chromosome Xp22.3-Xp21.3 as one of the best characterized large regions in the human genome. The map should greatly facilitate finer scale mapping and sequencing as well as the identification of disease genes from this portion of the human genome.

Citations

May 29, 2002·Human Gene Therapy·Maria Vittoria SchiaffinoMichele De Luca
Oct 29, 1997·Proceedings of the National Academy of Sciences of the United States of America·S PalmerA Ashworth
Jul 1, 1997·Mammalian Genome : Official Journal of the International Mammalian Genome Society·E Van de VosseJ T Den Dunnen
Sep 16, 1999·American Journal of Human Genetics·M M RyanK N North
Aug 12, 1999·American Journal of Human Genetics·L M BrzustowiczR Weksberg
Oct 27, 1997·American Journal of Human Genetics·R ScozzariA Novelletto
Jul 23, 1998·American Journal of Medical Genetics·V NarayananH Y Zoghbi
Sep 5, 2003·American Journal of Medical Genetics. Part a·Kym M BoycottPatrick M MacLeod
Feb 14, 2006·American Journal of Medical Genetics. Part a·Sylwia ChocholskaHildegard Kehrer-Sawatzki
Sep 22, 2000·The Journal of Clinical Endocrinology and Metabolism·S MummM P Whyte
Jun 4, 1998·Clinical Genetics·V des PortesM L Moutard
May 8, 1998·Methods : a Companion to Methods in Enzymology·G E Herman
Oct 1, 1996·Genome Research·H Roest CrolliusH Lehrach

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