An integrated proteomics and transcriptomics reference data set provides new insights into the Bradyrhizobium japonicum bacteroid metabolism in soybean root nodules

Proteomics
Nathanaël DelmotteGabriella Pessi

Abstract

Bradyrhizobium japonicum, a gram-negative soil bacterium that establishes an N(2)-fixing symbiosis with its legume host soybean (Glycine max), has been used as a symbiosis model system. Using a sensitive geLC-MS/MS proteomics approach, we report the identification of 2315 B. japonicum strain USDA110 proteins (27.8% of the theoretical proteome) that are expressed 21 days post infection in symbiosis with soybean cultivated in growth chambers, substantially expanding the previously known symbiosis proteome. Integration of transcriptomics data generated under the same conditions (2780 expressed genes) allowed us to compile a comprehensive expression profile of B. japonicum during soybean symbiosis, which comprises 3587 genes/proteins (43% of the predicted B. japonicum genes/proteins). Analysis of this data set revealed both the biases and the complementarity of these global profiling technologies. A functional classification and pathway analysis showed that most of the proteins involved in carbon and nitrogen metabolism are expressed, including a complete set of tricarboxylic acid cycle enzymes, several gluconeogenesis and pentose phosphate pathway enzymes, as well as several proteins that were previously not considered to be prese...Continue Reading

References

Aug 1, 1983·Archives of Microbiology·B Regensburger, H Hennecke
May 1, 1997·Molecular Plant-microbe Interactions : MPMI·N GuerreiroM A Djordjevic
Mar 5, 1999·Molecular Plant-microbe Interactions : MPMI·T WinzerP Müller
Aug 14, 1999·European Journal of Biochemistry·M MünchbachP James
May 2, 2000·Applied and Environmental Microbiology·M J LoriteE J Bedmar
Jul 25, 2000·Advances in Microbial Physiology·P Poole, D Allaway
Sep 7, 2000·Molecular Plant-microbe Interactions : MPMI·S H NateraM A Djordjevic
Oct 6, 2000·Annual Review of Microbiology·H P Spaink
Jan 12, 2001·Plant Physiology·S R Long
Feb 25, 2003·DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes·Takakazu KanekoSatoshi Tabata
Feb 25, 2003·DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes·Takakazu KanekoSatoshi Tabata
Jun 11, 2003·Molecular Plant-microbe Interactions : MPMI·Michael A DjordjevicUNKNOWN Sinorhizobium DNA Sequencing Consortium
Nov 25, 2003·Analytical Chemistry·Alexey I NesvizhskiiRuedi Aebersold
Mar 6, 2004·Molecular Plant-microbe Interactions : MPMI·Anke BeckerJacques Batut
Jul 21, 2004·Nature Reviews. Microbiology·Ray Dixon, Daniel Kahn
Jan 13, 2006·Proceedings of the National Academy of Sciences of the United States of America·Norihito KanamoriJens Stougaard
Jan 19, 2006·Biochemical Society Transactions·S MesaH-M Fischer
Feb 7, 2006·Current Opinion in Plant Biology·Gary StaceyGregory D May
Mar 8, 2006·Trends in Microbiology·Juergen Prell, Philip Poole
Mar 23, 2006·Molecular & Cellular Proteomics : MCP·Susanne WolffDörte Becher
May 17, 2006·Applied Microbiology and Biotechnology·Maria A Trainer, Trevor C Charles
Jan 17, 2007·Nature Reviews. Microbiology·Gary M King, Carolyn F Weber
Jul 17, 2007·Nature Reviews. Microbiology·Kathryn M JonesGraham C Walker
Nov 6, 2007·Molecular Plant-microbe Interactions : MPMI·Gabriella PessiHauke Hennecke
May 1, 2008·Annual Review of Plant Biology·Giles E D Oldroyd, J Allan Downie
Nov 6, 2008·Annual Review of Genetics·Katherine E GibsonGraham C Walker
Dec 20, 2008·Journal of Biotechnology·Jana HempelThomas Patschkowski
Jul 15, 2009·Proceedings of the National Academy of Sciences of the United States of America·J PrellP S Poole
Sep 22, 2009·Trends in Microbiology·Catherine Masson-BoivinJacques Batut

❮ Previous
Next ❯

Citations

Jun 11, 2011·Journal of Proteome Research·Alberto ParadelaFrancisco García-del Portillo
Sep 27, 2012·The Journal of Biological Chemistry·Fabio ServentiHauke Hennecke
Aug 2, 2013·Molecular Plant-microbe Interactions : MPMI·Aurelien L CarlierLeo Eberl
May 15, 2013·Journal of Bacteriology·J Ignacio QuelasAníbal R Lodeiro
Sep 12, 2013·PloS One·Gabriella PessiLeo Eberl
Mar 5, 2014·International Journal of Molecular Sciences·Nathanael DelmotteBenjamin Gourion
Jan 3, 2012·Trends in Biotechnology·Peer M SchenkKemal Kazan
Aug 16, 2011·Current Opinion in Microbiology·Melanie J Filiatrault
Oct 31, 2014·Proteomics·Brett Chapman, Matthew Bellgard
Oct 4, 2011·Analytica Chimica Acta·Animesh AcharjeeChris Maliepaard
Sep 5, 2014·Archives of Insect Biochemistry and Physiology·Pu Yang, Xiao-Ming Chen
Apr 26, 2014·The Biochemical Journal·David W Emerich, Hari B Krishnan
May 20, 2015·Journal of Proteomics·Mami NambuMitsuyoshi Ueda
Jan 20, 2012·RNA Biology·Ramakanth MadhugiriElena Evguenieva-Hackenberg
Dec 20, 2011·International Journal of Food Microbiology·Gabriela Lopez-VelascoMonica A Ponder
Jun 28, 2016·Proteomics·Dhirendra KumarDebasis Dash
May 13, 2010·Molecular Plant-microbe Interactions : MPMI·Marion KochHauke Hennecke
Jul 25, 2013·Molecular & Cellular Proteomics : MCP·Dhirendra KumarDebasis Dash
Oct 14, 2017·Molecular Plant-microbe Interactions : MPMI·Kent N StrodtmanDavid W Emerich
Jan 28, 2014·Applied and Environmental Microbiology·Marion KochGabriella Pessi
Apr 5, 2015·Applied and Environmental Microbiology·Martina LardiGabriella Pessi
Sep 12, 2018·Canadian Journal of Microbiology·George C diCenzoAlessio Mengoni
Mar 13, 2019·FEMS Microbiology Letters·Gesiele Almeida Barros-CarvalhoMarie-Anne Van Sluys
Jun 4, 2020·Molecular Systems Biology·Carlos Eduardo Flores-TinocoBeat Christen

❮ Previous
Next ❯

Related Concepts

Related Feeds

Aphasia

Aphasia affects the ability to process language, including formulation and comprehension of language and speech, as well as the ability to read or write. Here is the latest research on aphasia.