journal cover

An integrated RNA and CRISPR/Cas toolkit for multiplexed synthetic circuits and endogenous gene regulation in human cells

bioRxiv

Apr 23, 2014

Lior NissimTimothy Lu

Abstract

RNA-based regulation, such as RNA interference, and CRISPR/Cas transcription factors (CRISPR-TFs), can enable scalable synthetic gene circuits and the modulation of endogenous networks but have yet to be integrated together. Here, we combined multiple mammalian RNA regulatory strategies...read more

Mentioned in this Paper

Transcript
Introns
Protein Helix
Gene Regulatory Networks
Gene Circuits
RNA
RNA Polymerase II
Transcription Factor
Transcription, Genetic
Csy4 endoribonuclease, Pseudomonas aeruginosa
3
19
Paper Details
References
  • References
  • Citations
  • finger pointing at paper

    References currently unavailable

    We're still populating references for this paper, please check back later.
  • References
  • Citations
  • quote and clock

    No citations available

    This paper may not have been cited yet.

Similar Papers Found In These Feeds

Gene Regulatory Networks

Gene regulatory networks are comprised of molecular regulators that interact with each other and other factors in a cell to regulate and modulate gene expression. Here is the latest research.

CRISPR Ribonucleases Deactivation

CRISPR-Cas system enables the editing of genes to create or correct mutations. This feed focuses on mechanisms that underlie deactivation of CRISPR ribonucleases. Here is the latest research.

Eukaryotic RNA Polymerase II

RNA polymerase II (RNAP II) is a 550 kDa multiprotein complex composed of 12 subunits. Here is the latest research on RNAP II and its role it regulating transcription and transcription-coupled recombination repair.

BioRxiv Preprints

BioRxiv is the preprint server for biology, operated by Cold Spring Harbor Laboratory. Here are the latest biology preprint articles from bioRxiv.

RNAi & Gene Silencing (Preprints)

RNA Interference (RNAi) pathway is involved in post-transcriptional gene silencing, transcriptional silencing and epigenetic silencing as well as its use as a tool for forward genetics and therapeutics. Discover the latest preprints on RNAi here.

CRISPR for Genome Editing

Genome editing technologies enable the editing of genes to create or correct mutations. Clustered regularly interspaced short palindromic repeats (CRISPR) are DNA sequences in the genome that are recognized and cleaved by CRISPR-associated proteins (Cas). Here is the latest research on the use of CRISPR-Cas system in gene editing.

Single Cell Atlas - Preprints

Single cell sequencing (scs) can be harnessed to acquire the genomes, transcriptomes and epigenomes from individual cells. Here is the latest preprints on Single Cell Atlas.

MicroRNAs & Open Reading Frames

MicroRNAs are small non-coding RNAs that modulate gene expression at post-transcriptional level, playing a crucial role in cell differentiation and development. Discover the latest research on MicroRNAs & how they target Open Reading Frames.

Ribozymes

Ribozymes are catalytic enzymes that are made entirely of ribonucleic acid. These enzymes cleave the complementary mRNA sequence and are being studied as potential therapies for some dominantly inherited diseases. Discover the latest research on ribozymes here.

Gene Regulatory Networks (Keystone)

Gene regulatory networks are comprised of molecular regulators that interact with each other and other factors in a cell to regulate and modulate gene expression. Here is the latest research.

CRISPR for Genome Editing (Preprints)

Genome editing technologies enable the editing of genes to create or correct mutations. Clustered regularly interspaced short palindromic repeats (CRISPR) are DNA sequences in the genome that are recognized and cleaved by CRISPR-associated proteins (Cas). Here are the latest preprints on the use of CRISPR-Cas system in gene editing.

Eukaryotic RNA Polymerase II (Keystone)

RNA polymerase II (RNAP II) is a 550 kDa multiprotein complex composed of 12 subunits. Here is the latest research on RNAP II and its role it regulating transcription and transcription-coupled recombination repair.

CRISPR Genome Editing & Therapy (Preprints)

CRISPR-Cas system enables the editing of genes to create or correct mutations. This feed focuses on the application of this system for gene editing and therapy in human diseases.

CRISPR (general)

Clustered regularly interspaced short palindromic repeats (CRISPR) are DNA sequences in the genome that are recognized and cleaved by CRISPR-associated proteins (Cas). CRISPR-Cas system enables the editing of genes to create or correct mutations. Discover the latest research on CRISPR here.

RNAi & Gene Silencing

RNA Interference (RNAi) pathway is involved in post-transcriptional gene silencing, transcriptional silencing and epigenetic silencing as well as its use as a tool for forward genetics and therapeutics. Discover the latest research here.

© 2020 Meta ULC. All rights reserved

An integrated RNA and CRISPR/Cas toolkit for multiplexed synthetic circuits and endogenous gene regulation in human cells

bioRxiv

Apr 23, 2014

Lior NissimTimothy Lu

PMID: 990004432

DOI: 10.1101/004432

Abstract

RNA-based regulation, such as RNA interference, and CRISPR/Cas transcription factors (CRISPR-TFs), can enable scalable synthetic gene circuits and the modulation of endogenous networks but have yet to be integrated together. Here, we combined multiple mammalian RNA regulatory strategies...read more

Mentioned in this Paper

Transcript
Introns
Protein Helix
Gene Regulatory Networks
Gene Circuits
RNA
RNA Polymerase II
Transcription Factor
Transcription, Genetic
Csy4 endoribonuclease, Pseudomonas aeruginosa
3
19

Similar Papers Found In These Feeds

Paper Details
References
  • References
  • Citations
  • finger pointing at paper

    References currently unavailable

    We're still populating references for this paper, please check back later.
  • References
  • Citations
  • quote and clock

    No citations available

    This paper may not have been cited yet.
/papers/an-integrated-rna-and-crisprcas-toolkit-for/990004432