An Integrated Transcriptomic and Proteomic Analysis Identifies Significant Novel Pathways for Henoch-Schönlein Purpura Nephritis Progression.

BioMed Research International
Biao XiePing Zhou

Abstract

Although Henoch-Schönlein purpura nephritis (HSPN) is characterized by glomerular deposition of aberrantly glycosylated immunoglobulin A1 (IgA1), the underlying mechanism of HSPN progression has not yet been completely elucidated. In this study, we integrated transcriptomic and proteomic analyses to explore the underlying mechanism of HSPN progression. RNA sequencing and tandem mass tag- (TMT-) based quantitative proteomics were used to gain serum transcriptomic and proteomic profiles of patients with different types of HSPN (3 × type 1, 3 × type 2, and 3 × type 3). Student's t-tests were performed to obtain the significance of the differential gene expression. The clusterProfiler package was used to conduct the functional annotation of the DEGs for both Gene Ontology terms and Kyoto Encyclopedia of Genes and Genomes pathways. A total of 2315 mRNAs and 30 proteins were differentially expressed between the different types of HSPN. 58 mRNAs and one protein changed continuously during HSPN development and are potential biomarkers for HSPN progression. The validation cohort (another 9 patients) confirmed the high-throughput results of the transcriptomic and proteomic analyses. A total of 385 significant pathways were related to HSP...Continue Reading

References

Sep 21, 2005·Clinical Science·Stephen A Bustin, Reinhold Mueller
Mar 27, 2007·Lancet·Frank T Saulsbury
Jan 30, 2010·Critical Care Medicine·Marcel Levi, Tom van der Poll
Sep 17, 2010·Molecular Medicine·Suhail AkhtarMashkoor A Choudhry
Mar 12, 2011·Clinical Journal of the American Society of Nephrology : CJASN·Jean-Claude Davin
Mar 30, 2012·Omics : a Journal of Integrative Biology·Guangchuang YuQing-Yu He
Apr 17, 2012·Cell·Mathieu Laplante, David M Sabatini
Oct 26, 2012·The Journal of Pathology·Takahisa KawakamiJeremy S Duffield
Dec 18, 2013·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Kiyomi KoikeSeiya Okuda
Jan 16, 2014·Autoimmunity Reviews·Yao-Hsu YangBor-Luen Chiang
Jul 30, 2014·Nature Reviews. Nephrology·Jean-Claude Davin, Rosanna Coppo
Nov 22, 2014·Current Opinion in Nephrology and Hypertension·Frank C Brosius, John Cijiang He
Feb 18, 2016·The Journal of Immunology : Official Journal of the American Association of Immunologists·Guoku HuXian-Ming Chen
Nov 4, 2016·Methods in Molecular Biology·Ximena LeightonMeera Srivastava
Mar 23, 2017·Nature Immunology·Alejandro V VillarinoJohn J O'Shea
May 10, 2017·Genome Biology·Yehudit HasinAldons Lusis
Jul 8, 2017·Journal of the Renin-angiotensin-aldosterone System : JRAAS·Yuanyuan XuXiaoyun Jiang
Nov 4, 2017·PLoS Computational Biology·Florian RohartKim-Anh Lê Cao
Jan 22, 2018·Autoimmunity Reviews·Raquel López-MejíasMiguel A González-Gay
Jan 23, 2018·Omics : a Journal of Integrative Biology·Sara RedenšekTanja Kunej
Aug 4, 2018·Journal of the American Society of Nephrology : JASN·Jianchun ChenRaymond C Harris
Jan 9, 2019·Biochemical and Biophysical Research Communications·Bodokhsuren TsogbadrakhKook-Hwan Oh
Jan 18, 2019·Experimental and Therapeutic Medicine·Shuang PangJun Zhang

❮ Previous
Next ❯

Methods Mentioned

BETA
proteomic profiling
biopsy
protein assay
RNA-seq

Software Mentioned

R
mixOmics
clusterProfiler
MaxQuant
Omics
circosPlot

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

© 2021 Meta ULC. All rights reserved