An integrative study on biologically recovered polyhydroxyalkanoates (PHAs) and simultaneous assessment of gut microbiome in yellow mealworm

Journal of Biotechnology
Su Yean OngKumar Sudesh

Abstract

Polyhydroxyalkanoates (PHAs) are produced in microbes as a source of carbon and energy storage. They are biodegradable and have properties similar to synthetic plastics, which make them an interesting alternative to petroleum-based plastics. In this study, a refined method of recovering PHA from Cupriavidus necator biomass was proposed by incorporating the use of the yellow mealworm (the larval phase of the mealworm beetle, Tenebrio molitor) as partial purification machinery, followed by washing of the fecal pellets with distilled water and sodium hydroxide. The PHA contents of the cells used in this study were 55wt% (produced from palm olein) and 60 wt% (produced from waste animal fats). The treatment of distilled water and NaOH further increased the purity of PHA to 94%. In parallel, analysis of the 16S rRNA metagenomic sequencing of the mealworm gut microbiome has revealed remarkable changes in the bacterial diversity, especially between the mealworms fed with cells produced from palm olein and waste animal fats. This biological recovery of PHA from cells is an attempt to move towards a green and sustainable process with the aim of reducing the use of harmful solvents and strong chemicals during polymer purification. The res...Continue Reading

Citations

Aug 11, 2018·Journal of Basic Microbiology·Mananya MartlaKumar Sudesh
Feb 7, 2018·Applied Microbiology and Biotechnology·Su Yean OngKumar Sudesh
Feb 9, 2019·Journal, Genetic Engineering & Biotechnology·Neetu IsraniSrividya Shivakumar
Sep 10, 2020·Frontiers in Bioengineering and Biotechnology·Moritz BartelsSebastian L Riedel
Apr 8, 2020·Frontiers in Bioengineering and Biotechnology·Arthy SurendranKumar Sudesh
Jun 15, 2020·Current Microbiology·Shengchen WangLi Yi
Oct 29, 2020·Current Microbiology·K GonzalezMara Cea
May 18, 2020·The Science of the Total Environment·Jiaping LiJiao Lv
Dec 15, 2020·The Science of the Total Environment·Ayesha Algade AmaduSufeng Wang
Jan 31, 2021·Bioresource Technology·Vipin Chandra KaliaJung-Kul Lee
Aug 29, 2021·Environmental Science and Pollution Research International·Paola SangiorgioSimona Errico

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