PMID: 12755842May 21, 2003Paper

An inter- and intra-laboratory comparison of breath ¹³CO₂analysis

Alimentary Pharmacology & Therapeutics
F PerriSergio Vigneri

Abstract

¹³C breath test analysis requires accurate ¹³CO₂measurements. To perform a multicentre study to evaluate the repeatability and reproducibility of breath ¹³CO₂analysis. Two series of 25 paired randomly coded tubes (each consisting of 23 ¹³CO₂-enriched breath samples and two samples of standard reference pure CO₂with certified δ ¹³C(PDB)) were sent to participating centres for ¹³CO₂measurement. Each series of tubes was analysed 10 days apart. The repeatability and reproducibility of ¹³C measurements was assessed by Mandel's k and h statistics. Twenty-two centres participated in the study: 18 showed good inter- and intra-laboratory variability, whilst four showed abnormally high inter- or intra-laboratory variability. Breath test results were also significantly affected by the accuracy of the ¹³C analytical procedures. A low accuracy of ¹³C measurements may significantly affect the results of breath tests, leading to inappropriate clinical decisions. Standardization of ¹³C analysis is required to guarantee optimal ¹³C measurements and accurate ¹³C breath test results.

References

Nov 1, 1989·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·P A MichaletzB H Lauterburg
Jan 1, 1984·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·K J BreenM L Mashford
Mar 1, 1996·The Journal of Laboratory and Clinical Medicine·J H HoekstraY F Ghoos
Mar 20, 1999·Gut·F Stellaard, B Geypens

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Citations

Dec 23, 2003·Alimentary Pharmacology & Therapeutics·K JonderkoB Blonska-Fajfrowska
Feb 27, 2004·Alimentary Pharmacology & Therapeutics·F Perri
Sep 1, 2007·Journal of Breath Research·Anil S Modak

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