An interactive physiological role of neuropeptide Y and galanin in pulsatile pituitary luteinizing hormone secretion

Endocrinology
B XuS P Kalra

Abstract

Both neuropeptide Y (NPY) and galanin (GAL) systems have been implicated in the excitatory regulation of pulsatile LH secretion in the ovariectomized rat. The present studies were designed to examine the possible interaction of these two neuropeptides in controlling episodic LH release by testing the effects of central infusion of antibodies (Ab) to NPY and GAL, alone or in combination; additional studies tested the effects of central administration of an antisense oligodeoxynucleotide (ODN) to NPY and GAL messenger RNA. Rats were ovariectomized, implanted with a cannula in the third ventricle, and used in experiments 2 weeks later. Central infusion, via Alzet osmotic minipumps, of IgG purified from an NPY Ab produced a dose-related suppression of pulsatile LH secretion. Although an Ab dilution of 1:10 was ineffective, a maximal inhibitory effect was obtained using an NPY Ab dilution of 1:1, which decreased the mean levels, pulse frequency, and pulse amplitude of LH. These parameters of episodic LH secretion were also significantly reduced by central injection of antisense NPY ODN compared to those in vehicle- or missense ODN-treated controls. Similar dose-related inhibitory effects on the parameters of LH secretion were seen a...Continue Reading

Citations

Feb 17, 2001·Biochemical and Biophysical Research Communications·M IraharaT Aono
Dec 28, 1999·Journal of Chemical Neuroanatomy·E ChaillouY Tillet
Apr 11, 2001·Obstetrics and Gynecology Clinics of North America·B N KalroS L Berga
Nov 7, 2000·The Journal of Comparative Neurology·W B MathiesonP E Neumann
May 9, 2015·Bioscience, Biotechnology, and Biochemistry·Mina FujitaniTaro Kishida
Aug 21, 2003·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·Vincenzo Cimini
Feb 13, 2001·Endocrine Reviews·E Terasawa, D L Fernandez
Aug 3, 2000·Gynecological Endocrinology : the Official Journal of the International Society of Gynecological Endocrinology·A MilewiczU Tworowska
Jul 10, 2021·Gynecological Endocrinology : the Official Journal of the International Society of Gynecological Endocrinology·Engin Yildirim, Umit Gorkem

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