An interlaboratory study of the reproducibility and relevance of Episkin, a reconstructed human epidermis, in the assessment of cosmetics irritancy

Toxicology in Vitro : an International Journal Published in Association with BIBRA
R RoguetX Pouradier Duteil

Abstract

Reconstructed epidermal models are particularly suited to assessing the tolerance of cosmetic and dermatological products in vitro. Their production in kit form makes them available for screening both raw ingredients and finished products since a large amount of material can be tested whatever their physicochemical properties. However, two conditions must first be fulfilled: they must give reproducible results and be relevant to data obtained in vivo. We tested the reproducibility of data obtained using the Episkin(R) model [cytotoxicity evaluated by the MTT conversion and the release of one of the most active proinflammatory mediator, interleukin 1alpha(ILalpha)] on different batches and in various research laboratories. After topical application of sodium dodecyl sulfate (SDS) the overall variability of the IC(50) results was 14% of the mean value. Within a given centre and a given batch, the coefficient of variation attributable to the dispersion between kits was 6% for the SDS IC(50) determination and 7% for IL1alpha release measurement. The results obtained with Episkin were then compared with data from primary human skin irritancy testing (48-hr occlusion test and clinical assessment) and rabbit irritancy evaluation (Drai...Continue Reading

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Citations

Apr 17, 2002·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·M K RobinsonJ H Fentem
Sep 1, 2000·International Journal of Pharmaceutics·M PonecM Mommaas
Mar 22, 2001·Toxicology in Vitro : an International Journal Published in Association with BIBRA·J H FentemP A Botham
Sep 11, 2002·Toxicology in Vitro : an International Journal Published in Association with BIBRA·C FallerR Roguet
Mar 15, 2000·Journal of Pharmacological and Toxicological Methods·M K RobinsonM A Perkins
May 28, 2008·International Journal of Cosmetic Science·R RoguetM H Teissier
Mar 31, 2010·Toxicology in Vitro : an International Journal Published in Association with BIBRA·T HuM J Aardema
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Dec 15, 2004·Alternatives to Laboratory Animals : ATLA·Ludwig A Hothorn
Nov 18, 2000·Annals of the New York Academy of Sciences·M K RobinsonM A Perkins
Oct 15, 2020·Journal of Cosmetic Dermatology·Xiao MaBo Wang

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