An intermediate-conductance Ca2+-activated K+ channel is important for secretion in pancreatic duct cells

American Journal of Physiology. Cell Physiology
Mikio HayashiIvana Novak

Abstract

Potassium channels play a vital role in maintaining the membrane potential and the driving force for anion secretion in epithelia. In pancreatic ducts, which secrete bicarbonate-rich fluid, the identity of K(+) channels has not been extensively investigated. In this study, we investigated the molecular basis of functional K(+) channels in rodent and human pancreatic ducts (Capan-1, PANC-1, and CFPAC-1) using molecular and electrophysiological techniques. RT-PCR analysis revealed mRNAs for KCNQ1, KCNH2, KCNH5, KCNT1, and KCNT2, as well as KCNN4 coding for the following channels: KVLQT1; HERG; EAG2; Slack; Slick; and an intermediate-conductance Ca(2+)-activated K(+) (IK) channel (K(Ca)3.1). The following functional studies were focused on the IK channel. 5,6-Dichloro-1-ethyl-1,3-dihydro-2H-benzimidazole-2-one (DC-EBIO), an activator of IK channel, increased equivalent short-circuit current (I(sc)) in Capan-1 monolayer, consistent with a secretory response. Clotrimazole, a blocker of IK channel, inhibited I(sc). IK channel blockers depolarized the membrane potential of cells in microperfused ducts dissected from rodent pancreas. Cell-attached patch-clamp single-channel recordings revealed IK channels with an average conductance of...Continue Reading

References

Nov 1, 1975·The Journal of Physiology·W A Sewell, J A Young
May 1, 1990·The Journal of Membrane Biology·M A GrayB E Argent
Aug 1, 1991·Pflügers Archiv : European journal of physiology·I Novak, R Greger
Nov 1, 1993·Pflügers Archiv : European journal of physiology·I Novak, C Pahl
May 1, 1996·The Journal of General Physiology·P S SpectorM C Sanguinetti
Apr 15, 1997·Proceedings of the National Academy of Sciences of the United States of America·W P YangM A Blanar
Oct 23, 1997·Proceedings of the National Academy of Sciences of the United States of America·T M IshiiJ Maylie
Oct 6, 1997·Proceedings of the National Academy of Sciences of the United States of America·W J JoinerL K Kaczmarek
Aug 26, 1998·Pflügers Archiv : European journal of physiology·M Pellegrino, M Pellegrini
Feb 20, 1999·The Journal of Biological Chemistry·C M FangerJ Aiyar
Aug 24, 1999·The Journal of Physiology·H IshiguroM C Steward
Nov 11, 1999·Pflügers Archiv : European journal of physiology·S J Kim, R Greger
Jan 5, 2000·The Journal of Biological Chemistry·A C GerlachD C Devor
Aug 18, 2001·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·T von HahnR Warth
Oct 23, 2001·American Journal of Physiology. Gastrointestinal and Liver Physiology·T GreeleyM Soleimani
Mar 13, 2002·Pflügers Archiv : European journal of physiology·R WarthS J Kim
May 25, 2002·The Journal of Membrane Biology·A Wulf, A Schwab
Oct 22, 2002·American Journal of Physiology. Cell Physiology·T TakahataT Ishikawa
Feb 28, 2003·American Journal of Physiology. Gastrointestinal and Liver Physiology·William J JoinerVazhaikkurichi M Rajendran
Apr 19, 2003·The Journal of Physiology·Morten GrunnetDan A Klaerke
Apr 25, 2003·American Journal of Physiology. Cell Physiology·Peying FongMichael A Gray
Feb 16, 2005·Annual Review of Physiology·Martin C StewardR Maynard Case
Aug 3, 2005·Pflügers Archiv : European journal of physiology·Susanne E HedeIvana Novak
Sep 1, 2005·Pflügers Archiv : European journal of physiology·Susanne UllrichFlorian Lang
Dec 20, 2005·The Journal of Membrane Biology·L A PardoW Stühmer
Feb 1, 2006·The Journal of General Physiology·Marc PaulaisJacques Teulon
May 12, 2006·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Celia M SantiLawrence Salkoff
Jul 16, 2008·Physiological Reviews·Dirk Heitzmann, Richard Warth
Aug 20, 2008·Nature Genetics·Kazuki YasudaMasato Kasuga
Jul 10, 2010·American Journal of Physiology. Gastrointestinal and Liver Physiology·N S Nanda KumarVazhaikkurichi M Rajendran
Oct 28, 2010·The Journal of Biological Chemistry·Ivana NovakSusanne E Hede

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Citations

Feb 25, 2014·Cell Calcium·Marcelo A CatalánJames E Melvin
Mar 12, 2016·Pflügers Archiv : European journal of physiology·M HayashiH Matsuda
Aug 22, 2013·Channels·Mikio Hayashi, Ivana Novak
Jul 31, 2014·Pflügers Archiv : European journal of physiology·Viktória VengloveczPéter Hegyi
Oct 19, 2016·Pflügers Archiv : European journal of physiology·B BonitoI Novak
Jul 9, 2014·Current Opinion in Gastroenterology·Rashmi Chandra, Rodger A Liddle
Feb 6, 2018·Pflügers Archiv : European journal of physiology·N AndhariaH Matsuda
Jan 26, 2016·The Korean Journal of Physiology & Pharmacology : Official Journal of the Korean Physiological Society and the Korean Society of Pharmacology·Ming Zhe YinSung Joon Kim
Sep 1, 2015·American Journal of Physiology. Gastrointestinal and Liver Physiology·Jonathan M NicholsAldebaran M Hofer
Nov 14, 2019·International Journal of Molecular Sciences·Mikio Hayashi
Feb 1, 2013·American Journal of Physiology. Cell Physiology·Jing WangIvana Novak
Nov 13, 2020·Frontiers in Pharmacology·Julie SchnipperHalima Ouadid-Ahidouch
Apr 13, 2021·Frontiers in Pharmacology·Verena HofschröerAlbrecht Schwab

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