PMID: 3753585Jan 17, 1986Paper

An internal signal sequence: the asialoglycoprotein receptor membrane anchor

Cell
M Spiess, H F Lodish

Abstract

The human asialoglycoprotein receptor H1 is anchored in the membrane by a single stretch of 20 hydrophobic amino acids; the hydrophilic amino terminus faces the cytoplasm, and the carboxyl terminus is exoplasmic. We show here that glycosylation and insertion of the asialoglycoprotein receptor into the endoplasmic reticulum membrane is cotranslational and SRP-dependent and occurs without proteolytic cleavage. The membrane-anchor domain is necessary for membrane insertion, since a receptor with the segment deleted is neither inserted nor glycosylated. The segment is also sufficient for membrane insertion, since it will initiate translocation of a carboxy-terminal domain of rat alpha-tubulin across the membrane. We propose that a helical hairpin mechanism of membrane insertion is used both by cleaved amino-terminal and uncleaved internal signal sequences.

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Citations

May 1, 1990·The Journal of Membrane Biology·K Geering
Jun 18, 2009·Journal of Neural Transmission·Debra ToiberHermona Soreq
Oct 10, 1991·Biochimica Et Biophysica Acta·R G Shatters, J A Miernyk
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May 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·M A BourdonE Ruoslahti
Aug 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·Y AudigierG Blobel
Apr 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·S J SingerM P Yaffe
Oct 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·A S ShawJ K Rose
Feb 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·E Szczesna-SkorupaB Kemper
Aug 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·E HartmannH F Lodish
Sep 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·J B Ulmer, G E Palade
Oct 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·I GeffenM Spiess
Jan 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·M SakaguchiT Omura
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Oct 1, 1994·The Journal of Cell Biology·M M FalkN B Gilula
Sep 26, 2013·Molecular and Cellular Biology·Harvey Lodish
Jan 20, 2005·Gene·Stefan StammHermona Soreq

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