PMID: 8603913Mar 1, 1996Paper

An internalization signal in the simian immunodeficiency virus transmembrane protein cytoplasmic domain modulates expression of envelope glycoproteins on the cell surface

The Journal of Cell Biology
M M SauterJames A Hoxie

Abstract

A Tyr to Cys mutation at amino acid position 723 in the cytoplasmic domain of the simian immunodeficiency virus (SIV) transmembrane (TM) molecule has been shown to increase expression of envelope glycoproteins on the surface of infected cells. Here we show that Tyr-723 contributes to a sorting signal that directs the rapid endocytosis of viral glycoproteins from the plasma membrane via coated pits. On cells infected by SIVs with a Tyr at position 723, envelope glycoproteins were transiently expressed on the cell surface and then rapidly endocytosed. Similar findings were noted for envelope molecules expressed in the absence of other viral proteins. Immunoelectron microscopy demonstrated that these molecules were localized in patches on the cell surface and were frequently associated with coated pits. In contrast, envelope glycoproteins containing a Y723C mutation were diffusely distributed over the entire plasma membrane. To determine if an internalization signal was present in the SIV TM, chimeric molecules were constructed that contained the CD4 external and membrane spanning domains and a SIV TM cytoplasmic tail with a Tyr or other amino acids at SIV position 723. In Hela cells stably expressing these molecules, chimeras wit...Continue Reading

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Citations

May 23, 2001·Antiviral Research·C C LaBrancheS M Hammer
Jun 10, 1997·AIDS Research and Human Retroviruses·J Moore, A Trkola
Apr 18, 1998·AIDS Research and Human Retroviruses·B L ShacklettP Sonigo
Mar 26, 2011·AIDS Research and Human Retroviruses·Andrei N Vzorov, Richard W Compans
Oct 19, 2012·Journal of Virology·Thomas S Postler, Ronald C Desrosiers
Aug 10, 2000·Journal of Virology·Y E Lu, M Kielian
Jan 29, 2003·Journal of Virology·Wendy MaurySarahann Bradley
Feb 26, 2005·Journal of Virology·Carola VogtAndrea Maisner
Jan 1, 1996·Annual Review of Cell and Developmental Biology·L D HernandezJ M White

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