An intramolecular catalytic hairpin assembly on a DNA tetrahedron for mRNA imaging in living cells: improving reaction kinetics and signal stability.

Chemical Science
Zhihe QingRonghua Yang

Abstract

Enzyme-free amplification techniques based on dynamic DNA self-assembly (DDSA) have recently been developed for the in situ detection of mRNA in living cells. However, signal generation in traditional DDSA amplifiers is mainly dependent on the random diffusion of dissociative probes in a bulk solution, which is generally accompanied by poor kinetics and interference from complex biological systems. In this work, a new amplifier based on the design of an intramolecular catalytic hairpin assembly (intra-CHA) is proposed for the FRET imaging of mRNA in living cells. Compared with that in the free catalytic hairpin assembly (free-CHA), probes H1 and H2 in intra-CHA were simultaneously fixed on a DNA tetrahedron. The distance between them was closer, the local concentration of H1 and H2 in intra-CHA was theoretically approximately 808-times higher than that in free-CHA, and the initial reaction rate was enhanced 15.6 fold. Due to the spatial confinement effect, the reaction kinetics for target-catalyzed signal generation were significantly improved. By virtue of the three-dimensional nanostructure, H1 and H2 in the intra-CHA amplifier entered cells without any transfection or nanocarrier, and the probes and their products were free ...Continue Reading

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Citations

Jul 4, 2020·Chemical Communications : Chem Comm·Han WuJian-Hui Jiang
Aug 2, 2020·Biosensors & Bioelectronics·Yong ZhaoYun Peng
Aug 11, 2020·Biosensors & Bioelectronics·Zixin JiangZhihe Qing
Aug 22, 2020·Chemical Communications : Chem Comm·Jiaoli WangKemin Wang
Mar 2, 2021·Chemical Communications : Chem Comm·Chao XingChunhua Lu
Mar 18, 2021·Chemical Society Reviews·Fan LiXiaolei Zuo
Jul 1, 2021·Analytical Methods : Advancing Methods and Applications·Xu-Hao SunZhao-E Liu
Dec 17, 2020·Journal of the American Chemical Society·Liang YueItamar Willner
Sep 17, 2021·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Liuting MoWeiying Lin
Sep 22, 2021·Angewandte Chemie·Dong WangDan Luo
Nov 16, 2021·Luminescence : the Journal of Biological and Chemical Luminescence·Yalei LiuYufang Hu

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Methods Mentioned

BETA
transfection
fluorescence resonance
FRET
Fluorescence
fluorescence imaging
electrophoresis

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